Perreault C, Pelletier M, Belanger R, Boileau J, Bonny Y, David M, Gyger M, Landry D, Montplaisir S
Br J Haematol. 1985 Jun;60(2):253-60. doi: 10.1111/j.1365-2141.1985.tb07411.x.
Langerhans cells (LC) are bone marrow-derived dendritic antigen-presenting cells found in the epidermis. In an effort to determine the origin (host versus donor) of LC at different intervals following bone marrow transplantation, we performed skin biopsies in 16 recipients of sex-mismatched marrow. LC were identified using monoclonal antibody OKT6 in an indirect immunoperoxidase assay and their donor or host origin determined according to the presence or absence of Y body. The presence of Y-positive (donor) LC could be demonstrated in all (6/6) skin biopsies of female recipients of male marrow tested between days 39 and 730 post-transplant. Persistence of host LC in male recipients of female marrow was documented in all (6/6) recipients studied on day 39 and in two out of seven patients tested on day 120 post-transplant. From day 365 onward, no residual host LC could be detected, suggesting that by this time all epidermal LC are donor-derived. Our study demonstrates that host LC usually persist for 39 and up to 120 d following bone marrow transplantation. The relevance of this observation to the possible role of LC and other host dendritic antigen-presenting cells in the graft-versus-host reaction is discussed.
朗格汉斯细胞（LC）是存在于表皮中的源自骨髓的树突状抗原呈递细胞。为了确定骨髓移植后不同时间间隔的LC的起源（宿主还是供体），我们对16例性别不匹配的骨髓移植受者进行了皮肤活检。在间接免疫过氧化物酶试验中使用单克隆抗体OKT6鉴定LC，并根据Y小体的有无确定其供体或宿主来源。在移植后39天至730天之间测试的所有（6/6）男性骨髓女性受者的皮肤活检中，均可证明存在Y阳性（供体）LC。在移植后第39天研究的所有（6/6）受者以及移植后第120天测试的7例患者中的2例中，记录到女性骨髓男性受者中宿主LC的持续存在。从第365天起，未检测到残留的宿主LC，这表明此时所有表皮LC均来自供体。我们的研究表明，宿主LC通常在骨髓移植后持续39天至120天。讨论了这一观察结果与LC和其他宿主树突状抗原呈递细胞在移植物抗宿主反应中可能作用的相关性。
