Murphy G F, Merot Y, Tong A K, Smith B, Mihm M C
J Invest Dermatol. 1985 Mar;84(3):210-4. doi: 10.1111/1523-1747.ep12265149.
We have observed marked depletion of epidermal dendritic cells, defined by monoclonal antibodies directed against HLA-DR (Ia-like) and T6 antigens, after allogeneic bone marrow transplantation. To more precisely characterize this observation, we examined a total of 39 sequential biopsies from 15 patients both before and after allogeneic bone marrow transplantation. Profound depletion of HLA-DR and T6-positive epidermal dendritic cells was observed early after transplantation (1-4 weeks), followed by gradual and variable repopulation. Transmission electron microscopy confirmed absence of dendritic cells in selected biopsies. Depletion of dendritic cells did not appear to be related to development of clinical or histologic evidence of graft-versus-host disease, suggesting that depletion may relate to pretransplant conditioning regimens. The rate of return of these cells, however, may be influenced by the presence or persistence of clinical disease. Repopulation of epidermal dendritic cells after initial depletion in bone marrow transplantation represents a human model relevant to studies concerned with the origin and kinetics of Langerhans cells.
我们观察到，在同种异体骨髓移植后，由针对HLA - DR（类Ia）和T6抗原的单克隆抗体所定义的表皮树突状细胞显著减少。为了更精确地描述这一观察结果，我们检查了15例患者在同种异体骨髓移植前后共39份连续活检样本。移植后早期（1 - 4周）观察到HLA - DR和T6阳性表皮树突状细胞显著减少，随后逐渐且不同程度地重新填充。透射电子显微镜证实了在选定的活检样本中不存在树突状细胞。树突状细胞的减少似乎与移植物抗宿主病的临床或组织学证据的发展无关，这表明减少可能与移植前的预处理方案有关。然而，这些细胞的恢复速率可能受临床疾病的存在或持续情况影响。骨髓移植中初始减少后表皮树突状细胞的重新填充代表了一种与朗格汉斯细胞的起源和动力学研究相关的人体模型。
