The first department of Hepatobiliary and Pancreatic Surgery, Cangzhou Central Hospital, Cangzhou, China.
The first department of Hepatobiliary and Pancreatic Surgery, Cangzhou Central Hospital, Cangzhou, China.
Toxicology. 2024 Aug;506:153866. doi: 10.1016/j.tox.2024.153866. Epub 2024 Jun 21.
Tetrabromobisphenol S (TBBPS) is a brominated flame retardants (BFRs). TBBPS is widely used as a new type of BFR to replace TBBPA. Here, we used gastric cells as a model for evaluating the effect of TBBPS on the toxicology of gastric cells. Biochemical assays such as indirect immunofluorescence, cell proliferation assay were performed to analyze the toxicological effects of TBBPS on gastric cells. Cell proliferation analysis showed that TBBPS caused inhibition of gastric cell proliferation, and TBBPS induced gastric cell death. Further analysis showed that TBBPS led to ferroptosis and senescence of gastric cells by detecting ferroptosis-related marker molecules. Further work showed that TBBPS treatment resulted in lowered ferritin expression alongside heightened transferrin levels, which may be a potential molecular mechanism for TBBPS-induced ferroptosis and senescence in gastric cells. Here, our team investigates the effects of TBBPS on gastric cells in an in vitro model, and found that TBBPS caused toxicological damage to gastric cells. This study indicates potential toxic effects of TBBPS on the gastric cells, thereby providing a basis for further research into the toxicology of TBBPS.
四溴双酚 S（TBBPS）是一种溴化阻燃剂（BFRs）。TBBPS 被广泛用作一种新型 BFR 来替代 TBBPA。在这里，我们使用胃细胞作为模型来评估 TBBPS 对胃细胞毒理学的影响。通过间接免疫荧光、细胞增殖试验等生物化学方法分析 TBBPS 对胃细胞的毒性作用。细胞增殖分析表明，TBBPS 抑制胃细胞增殖，诱导胃细胞死亡。进一步分析表明，通过检测铁死亡相关标记分子，TBBPS 导致胃细胞发生铁死亡和衰老。进一步的工作表明，TBBPS 处理导致铁蛋白表达降低，转铁蛋白水平升高，这可能是 TBBPS 诱导胃细胞铁死亡和衰老的潜在分子机制。在这里，我们的团队在体外模型中研究了 TBBPS 对胃细胞的影响，发现 TBBPS 对胃细胞造成了毒理学损伤。这项研究表明 TBBPS 对胃细胞可能存在潜在的毒性作用，从而为进一步研究 TBBPS 的毒理学提供了依据。
