Ding Yuling, Dong Xing, Feng Weiwei, Mao Guanghua, Chen Yao, Qiu Xuchun, Chen Kun, Xu Hai
Institute of Environmental Health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Institute of Environmental Health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Sci Total Environ. 2022 Feb 1;806(Pt 1):150543. doi: 10.1016/j.scitotenv.2021.150543. Epub 2021 Sep 24.
Tetrabromobisphenol S (TBBPS), an emerging brominated flame retardant (BFR) has been widely detected in the environment, and may potentially pose environmental risks. However, data on the occurrence and toxic effects of TBBPS are limited. Circadian rhythms govern multiple behavioral and physiological processes, and their disruption is closely associated with various pathological conditions. Little is known about the potential for TBBPS to perturb circadian rhythm networks or circadian-driven locomotor behavior. In the present study, behavior assays and gene expression analysis based on circadian rhythm pathways were designed to investigate the potential circadian rhythm impairments and subsequent adverse effects caused in 120 h post-fertilization (hpf) zebrafish larvae by TBBPS. The development of embryos was inhibited by TBBPS exposure even at concentrations below the maximal non-lethal concentration (MNLC, 3.47 mg/L). Our results indicated remarkable alterations in the expression of several key circadian rhythm genes due to TBBPS exposure. Compared to control, the expression of per1a, per1b, per3, cry2, and csnk1da was increased, while the expression of clocka, clockb, cry4, cry1ba, arntl1a, and cank1db was decreased. Significant alterations of the circadian rhythm network could be observed in the zebrafish embryos. TBBPS exposure also significantly affected the behavioral responses of larvae. Our findings suggest the circadian rhythm network could be a potential target of TBBPS. Further study is needed to explore whether the transcriptional alterations in circadian rhythm translate into physiological effects.
四溴双酚S（TBBPS）是一种新兴的溴化阻燃剂（BFR），已在环境中被广泛检测到，并可能带来环境风险。然而，关于TBBPS的存在情况和毒性效应的数据有限。昼夜节律控制着多种行为和生理过程，其紊乱与各种病理状况密切相关。关于TBBPS扰乱昼夜节律网络或昼夜驱动的运动行为的可能性知之甚少。在本研究中，设计了基于昼夜节律途径的行为分析和基因表达分析，以研究TBBPS对受精后120小时（hpf）斑马鱼幼体造成的潜在昼夜节律损伤及后续不良影响。即使在低于最大非致死浓度（MNLC，3.47毫克/升）的浓度下，TBBPS暴露也会抑制胚胎发育。我们的结果表明，由于TBBPS暴露，几个关键昼夜节律基因的表达发生了显著变化。与对照组相比，per1a、per1b、per3、cry2和csnk1da的表达增加，而clocka、clockb、cry4、cry1ba、arntl1a和cank1db的表达降低。在斑马鱼胚胎中可观察到昼夜节律网络的显著变化。TBBPS暴露还显著影响了幼体的行为反应。我们的研究结果表明，昼夜节律网络可能是TBBPS的一个潜在靶点。需要进一步研究来探索昼夜节律中的转录变化是否会转化为生理效应。
