Ren Yanli, Sun Yunan, Javad Hafiz Umer, Wang Renkai, Zhou Zhiqing, Huang Yunmao, Shu Xugang, Li Cuijin
Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center, Jieyang, China.
College of Chemistry and Chemical Engineering, Zhongkai University of Agricultural Engineering, Guangzhou, China.
Biol Trace Elem Res. 2025 Feb;203(2):1035-1047. doi: 10.1007/s12011-024-04220-6. Epub 2024 Jun 24.
The aim of this study was to investigate the in vitro antioxidant activity of zinc ascorbate (AsA-Zn), its effects on the growth performance of and liver function in Magang geese under heat stress, and its potential mechanism. At AsA-Zn concentrations of 7.5, 15, 30, and 60 µmol/L, the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS·) radical scavenging rate increased significantly by 120.85%, 53.43%, 36.12%, and 0.99%, respectively, compared with that of ascorbic acid (AsA), indicating that AsA-Zn had better antioxidant performance in vitro. In this study, Magang geese were divided into a control group (basal diet, CON) and experimental groups, who received the basal diet supplemented with 400 mg/kg AsA or 30 (AsA-Zn30), 60 (AsA-Zn60), or 90 (AsA-Zn90) mg/kg AsA-Zn. AsA-Zn supplementation considerably reduced the feed-to-gain ratio, whereas both AsA and AsA-Zn significantly increased the thymus index. Moreover, AsA-Zn supplementation improved serum protein levels, lipid metabolism, liver function, and antioxidant capacity while reducing hepatocyte vacuolar degeneration. Furthermore, supplementation with AsA-Zn60 significantly increased the total antioxidant capacity, glutathione peroxidase activity, and superoxide dismutase activity and decreased the malondialdehyde content in the serum, liver, and hepatic mitochondria (P < 0.05), with more pronounced effects in the AsA-Zn60 group. Moreover, supplementation with ASA-Zn regulated the Nrf 2 signaling pathway and significantly increased the expression of genes encoding antioxidant-related factors in the liver. In conclusion, AsA-Zn has good antioxidant activity, and AsA-Zn supplementation may improve the antioxidant capacity of heat-stressed geese and promote their growth. Supplementation with 30 mg/kg AsA-Zn is recommended.
本研究旨在探讨抗坏血酸锌（AsA-Zn）的体外抗氧化活性、其对热应激下麻岗鹅生长性能和肝功能的影响及其潜在机制。在抗坏血酸锌浓度为7.5、15、30和60 μmol/L时，与抗坏血酸（AsA）相比，2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS·）自由基清除率分别显著提高了120.85%、53.43%、36.12%和0.99%，表明抗坏血酸锌在体外具有更好的抗氧化性能。在本研究中，麻岗鹅分为对照组（基础日粮，CON）和实验组，实验组接受补充400 mg/kg抗坏血酸或30（AsA-Zn30）、60（AsA-Zn60）或90（AsA-Zn90）mg/kg抗坏血酸锌的基础日粮。补充抗坏血酸锌显著降低了料重比，而抗坏血酸和抗坏血酸锌均显著提高了胸腺指数。此外，补充抗坏血酸锌改善了血清蛋白水平、脂质代谢、肝功能和抗氧化能力，同时减少了肝细胞空泡变性。此外，补充60 mg/kg抗坏血酸锌显著提高了血清、肝脏和肝线粒体中的总抗氧化能力、谷胱甘肽过氧化物酶活性和超氧化物歧化酶活性，并降低了丙二醛含量（P < 0.05），在AsA-Zn60组中效果更明显。此外，补充抗坏血酸锌调节了Nrf 2信号通路，并显著提高了肝脏中抗氧化相关因子编码基因的表达。总之，抗坏血酸锌具有良好的抗氧化活性，补充抗坏血酸锌可能提高热应激鹅的抗氧化能力并促进其生长。建议补充30 mg/kg抗坏血酸锌。
