College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, P. R. China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu Province 225009, P. R. China.
Poult Sci. 2019 Sep 1;98(9):3763-3769. doi: 10.3382/ps/pez066.
A total of 200 healthy 28-day-old male Jiangnan White geese were used to investigate the free radical scavenging ability, reduced glutathione (GSH) and thioredoxin systems, and the concentrations of reactive oxygen metabolites (ROM), malondialdehyde (MDA), and protein carbonyl (PC) in geese fed diets with organic selenium (Se) (Selenomethionine, SeMet) and inorganic Se (sodium selenite, SS). All geese were randomly allotted into 4 groups with 5 replicates of 10 geese each, and received basal diet supplemented with 0.3 mg Se/kg SS, 0.2, 0.3 and 0.4 mg Se/kg SeMet until 70 D of age, respectively. Geese in the SS and SeMet groups exhibited similar growth performance. Diet with SeMet increased the scavenging abilities of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical (ABTS•+, P < 0.001) and superoxide radical (O2-•, P = 0.002) in the serum of geese, as well as the scavenging abilities of ABTS•+ (P = 0.023), hydroxyl radical (P = 0.009) and O2-• (P = 0.019) in the liver of geese. Compared to the SS group, SeMet increased hepatic GSH concentration (P = 0.002), the activities of glutathione peroxidase (P = 0.031), γ-glutamate cysteine ligase (P < 0.001), and thioredoxin reductase (P < 0.001), and decreased the concentrations of ROM, MDA, and PC in the serum and liver of geese (P < 0.05). In conclusion, dietary SeMet inclusion would be more effective than SS in increasing the antioxidant capacity of geese, possibly by improving GSH and thioredoxin systems, and 0.2 mg Se/kg SeMet in goose diet is recommended.
本研究旨在探讨有机硒（硒代蛋氨酸，SeMet）和无机硒（亚硒酸钠，SS）对 28 日龄雄性江南白鹅生长性能、血清和肝脏自由基清除能力、谷胱甘肽（GSH）和硫氧还蛋白系统以及活性氧代谢物（ROM）、丙二醛（MDA）和蛋白羰基（PC）浓度的影响。将 200 只健康的 28 日龄雄性江南白鹅随机分为 4 组，每组 5 个重复，每个重复 10 只鹅。各组分别饲喂基础日粮添加 0.3 mg Se/kg SS、0.2、0.3 和 0.4 mg Se/kg SeMet，直至 70 日龄。结果表明，与 SS 组相比，SeMet 组显著提高了血清和肝脏中 2,2-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基（ABTS•+，P < 0.001）和超氧自由基（O2-•，P = 0.002）的清除能力，显著提高了肝脏中 ABTS•+（P = 0.023）、羟自由基（P = 0.009）和 O2-•（P = 0.019）的清除能力。此外，与 SS 组相比，SeMet 组显著提高了肝脏 GSH 浓度（P = 0.002）、谷胱甘肽过氧化物酶（P = 0.031）、γ-谷氨酰半胱氨酸连接酶（P < 0.001）和硫氧还蛋白还原酶（P < 0.001）的活性，降低了血清和肝脏中 ROM、MDA 和 PC 的浓度（P < 0.05）。综上所述，与 SS 相比，日粮添加 SeMet 更能有效提高鹅的抗氧化能力，这可能是通过改善 GSH 和硫氧还蛋白系统实现的，推荐江南白鹅日粮中添加 0.2 mg Se/kg SeMet。
