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用于产生命中化合物和先导化合物的新兴无色谱高通量质谱技术

Emerging Chromatography-Free High-Throughput Mass Spectrometry Technologies for Generating Hits and Leads.

作者信息

Pu Fan, Elsen Nathaniel L, Williams Jon D

机构信息

Drug Discovery Science and Technology, AbbVie Inc., 1 North Waukegan Road, North Chicago, Illinois 60064, United States.

出版信息

ACS Med Chem Lett. 2020 Sep 21;11(11):2108-2113. doi: 10.1021/acsmedchemlett.0c00314. eCollection 2020 Nov 12.

DOI:10.1021/acsmedchemlett.0c00314
PMID:33214819
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7667647/
Abstract

Mass spectrometry (MS) detection can offer unmatched selectivity and sensitivity. The use of MS without chromatography greatly increases the throughput, making it suitable for high throughput screening. However, the trade-offs of direct MS detection need to be carefully evaluated along with the development of novel strategies to ensure successful implementation. In this review, we will discuss the pros and cons of chromatography-free MS and discuss some of the currently used and future technologies being investigated to enable high-throughput MS.

摘要

质谱（MS）检测能够提供无与伦比的选择性和灵敏度。不使用色谱法的质谱联用极大地提高了通量，使其适用于高通量筛选。然而，在开发新策略以确保成功实施的同时，需要仔细评估直接质谱检测的权衡之处。在本综述中，我们将讨论无色谱质谱的优缺点，并探讨一些目前正在使用以及正在研究的未来技术，以实现高通量质谱分析。