Moskophidis M, Müller F
Immun Infekt. 1985 May;13(3):91-8.
Due to uncharacteristic symptoms of central nervous system (CNS) alterations during Treponema pallidum (TP) infection, the exact diagnosis of neurosyphilis has become more and more difficult. Particularly, there is a lack of sufficiently specific and sensitive assays for the demonstration of intrathecal synthesis of TP-specific immunoglobulins. TP-specific IgG or IgM class antibodies were estimated quantitatively in serum and cerebrospinal fluid (CSF) of 302 syphilitic patients by a modified enzyme immunoassay (ELISA), and antibody concentrations were quantitated as ELISA units per mg total IgG or IgM. In 197 of 237 patients with neurosyphilis an intrathecal synthesis of TP-specific IgG or IgM could be demonstrated by a 3- to 450-fold higher antibody concentration in the CSF than in the corresponding serum. In contrast, 65 patients without CNS involvement on the infection did not show any difference in the antibody concentration of CSF and serum. Thus, the diagnosis of neurosyphilis respectively the exclusion of CNS participation on the TP infection on an immunological base has become possible.
由于梅毒螺旋体（TP）感染期间中枢神经系统（CNS）改变出现非典型症状，神经梅毒的准确诊断变得越来越困难。特别是，缺乏足够特异和敏感的检测方法来证明鞘内合成TP特异性免疫球蛋白。通过改良酶免疫测定法（ELISA）对302例梅毒患者的血清和脑脊液（CSF）中的TP特异性IgG或IgM类抗体进行定量，抗体浓度以每毫克总IgG或IgM的ELISA单位进行定量。在237例神经梅毒患者中的197例中，CSF中TP特异性IgG或IgM的鞘内合成可通过其抗体浓度比相应血清高3至450倍得到证实。相比之下，65例感染时无CNS受累的患者CSF和血清中的抗体浓度没有差异。因此，基于免疫学诊断神经梅毒或排除TP感染时CNS受累已成为可能。
