人类巨噬细胞激活因子（用于葡萄糖消耗）mRNA在非洲爪蟾卵母细胞中的翻译。

Translation of human macrophage activating factor (for glucose consumption) mRNA in Xenopus laevis oocytes.

作者信息

Ishii Y, Osada H, Kobayashi Y, Obinata M, Natori S, Osawa T

出版信息

Immunol Invest. 1985 Apr;14(2):95-103. doi: 10.3109/08820138509042004.

DOI:10.3109/08820138509042004

Abstract

Total messenger RNA was extracted from a human T cell hybridoma, clone H-E4-9, which strongly produced macrophage activating factor for glucose consumption (MAF-G). This messenger RNA gave rise to functional MAF-G when translated in Xenopus laevis oocytes. Isoelectric focusing of culture supernatants of the mRNA-microinjected oocytes and the H-E4-9 cells revealed that the former contained MAF-Gs with isoelectric points of pH 5.0 and 3.0 while the latter contained MAF-Gs with isoelectric points of pH 5.0 and pH 3.3. Sucrose density gradient centrifugation analysis showed that MAF-G mRNA prepared from H-E4-9 cells sedimented at about 11.5 S.

摘要

从人T细胞杂交瘤克隆H-E4-9中提取总信使核糖核酸（mRNA），该克隆能强烈产生促进葡萄糖消耗的巨噬细胞活化因子（MAF-G）。当这种信使核糖核酸在非洲爪蟾卵母细胞中翻译时，可产生有功能的MAF-G。对显微注射了mRNA的卵母细胞和H-E4-9细胞的培养上清液进行等电聚焦分析，结果显示前者含有等电点为pH 5.0和3.0的MAF-G，而后者含有等电点为pH 5.0和pH 3.3的MAF-G。蔗糖密度梯度离心分析表明，从H-E4-9细胞制备的MAF-G mRNA沉降系数约为11.5 S。