Developmental, Stem Cell and Cancer Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
Wageningen Bioveterinary Research, Wageningen University & Research, Lelystad, The Netherlands.
J Mammary Gland Biol Neoplasia. 2024 Jun 25;29(1):13. doi: 10.1007/s10911-024-09565-1.
Conflicting data exist as to how mammary epithelial cell proliferation changes during the reproductive cycle. To study the effect of endogenous hormone fluctuations on gene expression in the mouse mammary gland, we performed bulk RNAseq analyses of epithelial and stromal cell populations that were isolated either during puberty or at different stages of the adult virgin estrous cycle. Our data confirm prior findings that proliferative changes do not occur in every mouse in every cycle. We also show that during the estrous cycle the main gene expression changes occur in adipocytes and fibroblasts. Finally, we present a comprehensive overview of the Wnt gene expression landscape in different mammary gland cell types in pubertal and adult mice. This work contributes to understanding the effects of physiological hormone fluctuations and locally produced signaling molecules on gene expression changes in the mammary gland during the reproductive cycle and should be a useful resource for future studies investigating gene expression patterns in different cell types across different developmental timepoints.
关于乳腺上皮细胞增殖在生殖周期中如何变化，存在相互矛盾的数据。为了研究内源性激素波动对小鼠乳腺基因表达的影响，我们对青春期或成年雌性发情周期不同阶段分离的上皮细胞和基质细胞群体进行了批量 RNA 测序分析。我们的数据证实了之前的发现，即在每个周期中并非每个小鼠都会发生增殖变化。我们还表明，在发情周期中，主要的基因表达变化发生在脂肪细胞和成纤维细胞中。最后，我们展示了青春期和成年小鼠不同乳腺细胞类型中 Wnt 基因表达图谱的综合概述。这项工作有助于理解生理激素波动和局部产生的信号分子对生殖周期中乳腺基因表达变化的影响，并且应该是未来研究不同发育时间点不同细胞类型基因表达模式的有用资源。
