Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Fisiología, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Acta Odontol Latinoam. 2024 Apr;37(1):45-58. doi: 10.54589/aol.37/1/45.
Xerostomia emerges as a consequence of salivary gland hypofunction, and seriously compromises the integrity of hard and soft oral tissues, whileperiodontitis is an infectious disease characterized by biofilm accumulation, inflammation and alveolar bone resorption.
The aim this study was to compare the deleterious effects caused by experimental hyposalivation, periodontitis, and the combination of both on periodontal tissues and mandibular biomechanics in rats.
Hyposalivation (group H) was induced through bilateral submandibulectomy. Periodontitis (group EP) was induced by injecting LPS (1 mg/ml) into the gingiva of the first lower molars. A third group was subjected to both conditions (group H+EP). Alveolar bone loss was evaluated by micro-computed tomography and histomorphometric analysis, and gingival inflammatory mediators were assessed by specific techniques. Biomechanical properties were evaluated in mandible.
Alveolar bone loss increased similarly in groups H, EP and H+EP compared to control. Metalloproteinase (MMP2 and MMP9) activity was similar in H and control, but higher in groups EP and H+EP (MMP2: C 9644+2214, EP 34441+3336, H 5818+1532, H+EP 42673+3184; MMP9: C 5792+961, EP 14807+861, H 9295+520, H+EP 4838+1531). The rest of the inflammatory mediators evaluated increased in groups H, EP and H+EP to a greater or lesser extent with respect to the control, although in most cases, they were higher in groups EP and H+EP than in group H. The biomechanical properties of the mandible increased in group H compared to the other three groups.
Both hyposalivation and periodontitis cause periodontal damage, but hyposalivation also produces biomechanical alterations, causing more extensive deleterious effects than periodontitis.
本研究旨在比较实验性唾液腺功能低下、牙周炎和两者联合对大鼠牙周组织和下颌骨生物力学的有害影响。
通过双侧颌下腺切除术诱导唾液腺功能低下（组 H）。通过将 LPS（1mg/ml）注入第一下磨牙的牙龈诱导牙周炎（组 EP）。第三组接受两种条件（组 H+EP）。通过微计算机断层扫描和组织形态计量分析评估牙槽骨丧失，通过特定技术评估牙龈炎症介质。评估下颌骨的生物力学特性。
与对照组相比，组 H、EP 和 H+EP 的牙槽骨丧失均相似增加。基质金属蛋白酶（MMP2 和 MMP9）活性在 H 和对照组中相似，但在 EP 和 H+EP 组中更高（MMP2：C 9644+2214、EP 34441+3336、H 5818+1532、H+EP 42673+3184；MMP9：C 5792+961、EP 14807+861、H 9295+520、H+EP 4838+1531）。与对照组相比，评估的其余炎症介质在组 H、EP 和 H+EP 中或多或少地增加，但在大多数情况下，它们在 EP 和 H+EP 组中高于 H 组。与其他三组相比，下颌骨的生物力学特性在 H 组中增加。
唾液腺功能低下和牙周炎均可引起牙周损伤，但唾液腺功能低下还会引起生物力学改变，造成比牙周炎更广泛的有害影响。
