Botts J, Thomason J F, Morales M F
Cardiovascular Research Institute, University of California, San Francisco 94143.
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2204-8. doi: 10.1073/pnas.86.7.2204.
A proximity map showing the three-dimensional arrangement of 12 chemically defined points in actomyosin subfragment 1 is developed and roughly correlated with published electron microscope reconstruction of others. Several additional points and topological relationships in the primary polypeptide chain folding are assimilated into this model. Certain crosslinkings and distance change observations are interpreted as indicators of transmission of force/displacement between the nucleotide-binding and an actin-binding site--i.e., as indications of how energy is transduced in this system.
构建了一个显示肌动球蛋白亚片段1中12个化学定义点的三维排列的邻近图谱，并将其与已发表的其他人的电子显微镜重建结果大致关联起来。初级多肽链折叠中的几个额外的点和拓扑关系被纳入该模型。某些交联和距离变化观察结果被解释为核苷酸结合位点和肌动蛋白结合位点之间力/位移传递的指标，即作为该系统中能量如何转换的指示。
