Zhang Cong, Wei Guohua, Wu Linyuan, Zhang Yunhui, Zhu Xun, Merchant Austin, Zhou Xuguo, Liu Xiangying, Li Xiangrui
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Insects. 2024 Jun 19;15(6):459. doi: 10.3390/insects15060459.
The investigation of genetics-based biopesticides has become a central focus in pesticide studies due to their inherent advantages, including species specificity, environmental safety, and a wide range of target genes. In this study, a mixture of miR-184 agomir and nanomaterial star polycation (SPc) was used to treat the nymphs of the English grain aphid, (F.). The life parameters of the aphids at various developmental stages were analyzed using an age-stage two-sex life table to assess the effect of miR-184 agomir on the experimental population. The results indicated that miR-184 agomir had a significant negative effect on four key life parameters, including the intrinsic rate of increase, the finite rate of increase, the net rate of increase, and the mean generation time. The population prediction revealed a substantial reduction (91.81% and 95.88%) in the population size of at 60 d after treatment with miR-184 agomir, compared to the control groups. Our findings suggest that the miR-184 agomir has the potential to reduce the survival rate and mean longevity of , highlighting its potential as a promising candidate for the development of an effective genetics-based biopesticide.
基于遗传学的生物农药研究因其固有优势，包括物种特异性、环境安全性和广泛的靶基因，已成为农药研究的核心焦点。在本研究中，使用miR - 184激动剂和纳米材料星形聚阳离子（SPc）的混合物处理麦长管蚜（Sitobion avenae (F.)）若虫。利用年龄 - 龄期两性生命表分析蚜虫不同发育阶段的生命参数，以评估miR - 184激动剂对实验种群的影响。结果表明，miR - 184激动剂对四个关键生命参数有显著负面影响，包括内禀增长率、周限增长率、净增殖率和平均世代时间。种群预测显示，与对照组相比，用miR - 184激动剂处理60天后，麦长管蚜种群数量大幅减少（分别为91.81%和95.88%）。我们的研究结果表明，miR - 184激动剂有可能降低麦长管蚜的存活率和平均寿命，突出了其作为开发有效基于遗传学的生物农药的有前景候选物的潜力。
