• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于星形聚阳离子纳米载体的 miRNA 递释系统在拟南芥和玉米中的构建与应用。

Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize.

机构信息

State Key Laboratory of Plant Physiology and Biochemistry, Engineering Research Center of Plant Growth Regulator, Ministry of Education & College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.

Department of Plant Biosecurity and MARA Key Laboratory for Monitoring and Green Management, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.

出版信息

J Nanobiotechnology. 2022 May 7;20(1):219. doi: 10.1186/s12951-022-01443-4.

DOI:10.1186/s12951-022-01443-4
PMID:35525952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9077854/
Abstract

BACKGROUND

MicroRNA (miRNA) plays vital roles in the regulation of both plant architecture and stress resistance through cleavage or translation inhibition of the target messenger RNAs (mRNAs). However, miRNA-induced gene silencing remains a major challenge in vivo due to the low delivery efficiency and instability of miRNA, thus an efficient and simple method is urgently needed for miRNA transformation. Previous researches have constructed a star polycation (SPc)-mediated transdermal double-stranded RNA (dsRNA) delivery system, achieving efficient dsRNA delivery and gene silencing in insect pests.

RESULTS

Here, we tested SPc-based platform for direct delivery of double-stranded precursor miRNA (ds-MIRNA) into protoplasts and plants. The results showed that SPc could assemble with ds-MIRNA through electrostatic interaction to form nano-sized ds-MIRNA/SPc complex. The complex could penetrate the root cortex and be systematically transported through the vascular tissue in seedlings of Arabidopsis and maize. Meanwhile, the complex could up-regulate the expression of endocytosis-related genes in both protoplasts and plants to promote the cellular uptake. Furthermore, the SPc-delivered ds-MIRNA could efficiently increase mature miRNA amount to suppress the target gene expression, and the similar phenotypes of Arabidopsis and maize were observed compared to the transgenic plants overexpressing miRNA.

CONCLUSION

To our knowledge, we report the first construction and application of star polycation nanocarrier-based platform for miRNA delivery in plants, which explores a new enable approach of plant biotechnology with efficient transformation for agricultural application.

摘要

背景

微小 RNA（miRNA）通过靶信使 RNA（mRNA）的切割或翻译抑制，在植物结构和抗逆性的调控中发挥着重要作用。然而，由于 miRNA 的递送效率低和不稳定性，miRNA 诱导的基因沉默在体内仍然是一个主要挑战，因此迫切需要一种高效、简单的 miRNA 转化方法。先前的研究构建了一种星形聚阳离子（SPc）介导的透皮双链 RNA（dsRNA）递药系统，实现了昆虫中高效的 dsRNA 递药和基因沉默。

结果

在这里，我们测试了基于 SPc 的平台，用于直接将双链前体 miRNA（ds-MIRNA）递送至原生质体和植物中。结果表明，SPc 可以通过静电相互作用与 ds-MIRNA 组装形成纳米大小的 ds-MIRNA/SPc 复合物。该复合物可以穿透根皮层，并通过血管组织在拟南芥和玉米的幼苗中进行系统运输。同时，该复合物可以上调原生质体和植物中内吞相关基因的表达，以促进细胞摄取。此外，SPc 递送的 ds-MIRNA 可以有效地增加成熟 miRNA 的数量，从而抑制靶基因的表达，并观察到拟南芥和玉米表现出与过表达 miRNA 的转基因植物相似的表型。

结论

据我们所知，我们首次构建并应用了基于星形聚阳离子纳米载体的 miRNA 递药平台在植物中，这为农业应用的植物生物技术探索了一种新的有效转化方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/e50f1f7b1670/12951_2022_1443_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/b644efcac642/12951_2022_1443_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/2e4a24f048fc/12951_2022_1443_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/3528721279c6/12951_2022_1443_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/872e62bf6958/12951_2022_1443_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/b10b96b9a676/12951_2022_1443_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/7d4081861c97/12951_2022_1443_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/e50f1f7b1670/12951_2022_1443_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/b644efcac642/12951_2022_1443_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/2e4a24f048fc/12951_2022_1443_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/3528721279c6/12951_2022_1443_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/872e62bf6958/12951_2022_1443_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/b10b96b9a676/12951_2022_1443_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/7d4081861c97/12951_2022_1443_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7039/9077854/e50f1f7b1670/12951_2022_1443_Fig6_HTML.jpg

相似文献

1
Construction and application of star polycation nanocarrier-based microRNA delivery system in Arabidopsis and maize.基于星形聚阳离子纳米载体的 miRNA 递释系统在拟南芥和玉米中的构建与应用。
J Nanobiotechnology. 2022 May 7;20(1):219. doi: 10.1186/s12951-022-01443-4.
2
Visualization of the process of a nanocarrier-mediated gene delivery: stabilization, endocytosis and endosomal escape of genes for intracellular spreading.纳米载体介导基因传递过程的可视化：基因的稳定、内吞作用和内涵体逃逸，以实现细胞内扩散。
J Nanobiotechnology. 2022 Mar 9;20(1):124. doi: 10.1186/s12951-022-01336-6.
3
Identification of miRNAs and their target genes in developing maize ears by combined small RNA and degradome sequencing.通过联合小 RNA 和降解组测序鉴定玉米穗发育过程中的 miRNAs 和它们的靶基因。
BMC Genomics. 2014 Jan 14;15:25. doi: 10.1186/1471-2164-15-25.
4
Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomes.广泛存在的 miRNA 介导的膜结合多核糖体靶标切割。
Genome Biol. 2021 Jan 5;22(1):15. doi: 10.1186/s13059-020-02242-6.
5
Efficient silencing of endogenous microRNAs using artificial microRNAs in Arabidopsis thaliana.利用人工 microRNA 在拟南芥中高效沉默内源性 microRNAs。
Mol Plant. 2011 Jan;4(1):157-70. doi: 10.1093/mp/ssq061. Epub 2010 Oct 13.
6
Self-assembled co-delivery nanoplatform for increasing the broad-spectrum susceptibility of fall armyworm toward insecticides.用于提高草地贪夜蛾对杀虫剂广谱敏感性的自组装共递送纳米平台。
J Adv Res. 2025 Jan;67:93-104. doi: 10.1016/j.jare.2024.01.031. Epub 2024 Jan 28.
7
Ectopic DICER-LIKE1 expression in P1/HC-Pro Arabidopsis rescues phenotypic anomalies but not defects in microRNA and silencing pathways.在P1/HC-Pro拟南芥中异位表达DICER-LIKE1可挽救表型异常，但不能挽救微小RNA和沉默途径中的缺陷。
Plant Cell. 2005 Nov;17(11):2873-85. doi: 10.1105/tpc.105.036608. Epub 2005 Oct 7.
8
The use of artificial microRNA technology to control gene expression in Arabidopsis thaliana.利用人工微小RNA技术调控拟南芥中的基因表达。
Methods Mol Biol. 2014;1062:211-24. doi: 10.1007/978-1-62703-580-4_11.
9
miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea mays L.).miRNA164 靶向切割 ZmNAC1 赋予玉米（Zea mays L.）侧根发育。
BMC Plant Biol. 2012 Nov 21;12:220. doi: 10.1186/1471-2229-12-220.
10
A virus-based miRNA suppression (VbMS) system for miRNA loss-of-function analysis in plants.基于病毒的 miRNA 抑制（VbMS）系统，用于植物中的 miRNA 功能丧失分析。
Biotechnol J. 2014 May;9(5):702-8. doi: 10.1002/biot.201300523. Epub 2014 Apr 10.

引用本文的文献

1
Lipid membrane-camouflaged biomimetic nanoparticle for MicroRNA based therapeutic delivery to intestinal epithelial cells.用于基于微小RNA的治疗性递送至肠上皮细胞的脂质膜伪装仿生纳米颗粒。
Sci Rep. 2025 Aug 26;15(1):31363. doi: 10.1038/s41598-025-17382-7.
2
Nanocarrier-mediated transdermal delivery of Lmidgf4 dsRNA expedites biological control of locusts by Beauveria bassiana.纳米载体介导的Lmidgf4双链RNA经皮递送可加速球孢白僵菌对蝗虫的生物防治。
J Nanobiotechnology. 2025 Apr 4;23(1):272. doi: 10.1186/s12951-025-03347-5.
3
Nanocarrier-Based Eco-Friendly RNA Pesticides for Sustainable Management of Plant Pathogens and Pests.

本文引用的文献

1
Visualization of the process of a nanocarrier-mediated gene delivery: stabilization, endocytosis and endosomal escape of genes for intracellular spreading.纳米载体介导基因传递过程的可视化：基因的稳定、内吞作用和内涵体逃逸，以实现细胞内扩散。
J Nanobiotechnology. 2022 Mar 9;20(1):124. doi: 10.1186/s12951-022-01336-6.
2
Nanoparticle cellular internalization is not required for RNA delivery to mature plant leaves.纳米颗粒的细胞内化对于 RNA 递送到成熟植物叶片不是必需的。
Nat Nanotechnol. 2022 Feb;17(2):197-205. doi: 10.1038/s41565-021-01018-8. Epub 2021 Nov 22.
3
Nanocarrier-delivered dsRNA suppresses wing development of green peach aphids.
基于纳米载体的环保型RNA农药用于植物病原体和害虫的可持续治理
Nanomaterials (Basel). 2024 Nov 22;14(23):1874. doi: 10.3390/nano14231874.
4
Nanomaterial inactivates environmental virus and enhances plant immunity for controlling tobacco mosaic virus disease.纳米材料可灭活环境病毒并增强植物免疫力，从而控制烟草花叶病毒病。
Nat Commun. 2024 Oct 1;15(1):8509. doi: 10.1038/s41467-024-52851-z.
5
Utilizing Star Polycation Nanocarrier for the Delivery of miR-184 Agomir and Its Impact on the Life History Traits of the English Grain Aphid, .利用星型聚阳离子纳米载体递送miR-184激动剂及其对麦长管蚜生活史特征的影响
Insects. 2024 Jun 19;15(6):459. doi: 10.3390/insects15060459.
6
Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.通过纳米技术和跨领域 RNA 运输的见解来提高基于 RNA 的作物保护。
Curr Opin Plant Biol. 2023 Dec;76:102441. doi: 10.1016/j.pbi.2023.102441. Epub 2023 Sep 9.
7
Nanotechnology in Food and Plant Science: Challenges and Future Prospects.食品与植物科学中的纳米技术：挑战与未来展望
Plants (Basel). 2023 Jul 6;12(13):2565. doi: 10.3390/plants12132565.
8
Recent Advances in Nanoparticle-Mediated Co-Delivery System: A Promising Strategy in Medical and Agricultural Field.纳米粒子介导的共递系统的最新进展：医学和农业领域的有前途策略。
Int J Mol Sci. 2023 Mar 7;24(6):5121. doi: 10.3390/ijms24065121.
9
Calcium nutrition nanoagent rescues tomatoes from mosaic virus disease by accelerating calcium transport and activating antiviral immunity.钙营养纳米制剂通过加速钙转运和激活抗病毒免疫来拯救番茄免受花叶病毒病侵害。
Front Plant Sci. 2022 Dec 6;13:1092774. doi: 10.3389/fpls.2022.1092774. eCollection 2022.
10
A Preparation Method of Nano-Pesticide Improves the Selective Toxicity toward Natural Enemies.一种纳米农药的制备方法提高了对天敌的选择性毒性。
Nanomaterials (Basel). 2022 Jul 14;12(14):2419. doi: 10.3390/nano12142419.
纳米载体递送的双链 RNA 抑制了绿桃蚜的翅膀发育。
Insect Sci. 2022 Jun;29(3):669-682. doi: 10.1111/1744-7917.12953. Epub 2021 Oct 13.
4
Simple Osthole/Nanocarrier Pesticide Efficiently Controls Both Pests and Diseases Fulfilling the Need of Green Production of Strawberry.简单型蛇床子素/纳米载体农药高效防控草莓病虫害，满足草莓绿色生产需求。
ACS Appl Mater Interfaces. 2021 Aug 4;13(30):36350-36360. doi: 10.1021/acsami.1c09887. Epub 2021 Jul 20.
5
Gold-Nanocluster-Mediated Delivery of siRNA to Intact Plant Cells for Efficient Gene Knockdown.金纳米簇介导的 siRNA 递送至完整植物细胞以实现高效基因敲低。
Nano Lett. 2021 Jul 14;21(13):5859-5866. doi: 10.1021/acs.nanolett.1c01792. Epub 2021 Jun 21.
6
Nanometerization of thiamethoxam by a cationic star polymer nanocarrier efficiently enhances the contact and plant-uptake dependent stomach toxicity against green peach aphids.纳米化噻虫嗪通过阳离子星型聚合物纳米载体可有效提高其对绿盲蝽的接触和取食依赖的胃毒活性。
Pest Manag Sci. 2021 Apr;77(4):1954-1962. doi: 10.1002/ps.6223. Epub 2021 Jan 14.
7
Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown.碳纳米载体将 siRNA 递送至完整的植物细胞中，实现高效基因敲低。
Sci Adv. 2020 Jun 24;6(26):eaaz0495. doi: 10.1126/sciadv.aaz0495. eCollection 2020 Jun.
8
Differentially charged nanoplastics demonstrate distinct accumulation in Arabidopsis thaliana.带不同电荷的纳米塑料在拟南芥中表现出明显的积累差异。
Nat Nanotechnol. 2020 Sep;15(9):755-760. doi: 10.1038/s41565-020-0707-4. Epub 2020 Jun 22.
9
Nanoparticle-mediated double-stranded RNA delivery system: A promising approach for sustainable pest management.纳米颗粒介导的双链 RNA 递药系统：一种有前途的可持续害虫治理方法。
Insect Sci. 2021 Feb;28(1):21-34. doi: 10.1111/1744-7917.12822. Epub 2020 Jul 20.
10
MicroRNA-125a-Loaded Polymeric Nanoparticles Alleviate Systemic Lupus Erythematosus by Restoring Effector/Regulatory T Cells Balance.负载微小RNA-125a的聚合物纳米颗粒通过恢复效应/调节性T细胞平衡来缓解系统性红斑狼疮。
ACS Nano. 2020 Apr 28;14(4):4414-4429. doi: 10.1021/acsnano.9b09998. Epub 2020 Mar 26.