Frederick D L, Green J, Fowler M W
J Anal Toxicol. 1985 May-Jun;9(3):116-20. doi: 10.1093/jat/9.3.116.
Numerous methods for the detection of urinary metabolites of marijuana constituents are available. Documentation of the sensitivity and specificity of these tests is needed before the determination of a pair of screening-confirmation tests can be made. This study used 88 clinical specimens to evaluate five commercially available marijuana metabolite methods and one new gas chromatography/mass spectrometry (GC/MS) method. The EMIT-d.a.u. test was found to have 2 to 3% unconfirmed positives when compared to the other methods evaluated. The new thin layer procedure, TOXI-LAB, was not as sensitive as the EMIT-d.a.u. procedure for some specimens, but proved to be a good confirmation for the EMIT-d.a.u. with elimination of all "unconfirmed positives." The Abuscreen (Roche) and the EMIT-st assays were positive for samples that contained larger amounts of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (11-nor-delta 9-THC-9-COOH). The Immunalysis-radioimmunoassay (RIA) was positive for all samples found positive by the GC/MS method, but the concentrations found by the two assays did not correlate. The GC/MS method was developed to use the same extraction as the thin layer procedure and provides confirmation for all procedures except 2 to 3% of the positive EMIT-d.a.u. results.
检测大麻成分尿液代谢物的方法有很多种。在确定一对筛查 - 确证试验之前，需要记录这些检测方法的灵敏度和特异性。本研究使用了88份临床标本，以评估五种市售的大麻代谢物检测方法和一种新的气相色谱/质谱（GC/MS）方法。与其他评估方法相比，发现EMIT - d.a.u.检测有2%至3%的未确认阳性结果。新的薄层检测方法TOXI - LAB，对某些标本的灵敏度不如EMIT - d.a.u.检测方法，但被证明是EMIT - d.a.u.检测的良好确证方法，能消除所有“未确认阳性”。Abuscreen（罗氏公司）和EMIT - st检测对含有大量11 - 去甲 - Δ9 - 四氢大麻酚 - 9 - 羧酸（11 - nor - Δ9 - THC - 9 - COOH）的样本呈阳性。免疫分析放射免疫测定（RIA）对所有经GC/MS方法检测为阳性的样本均呈阳性，但两种检测方法测得的浓度不相关。GC/MS方法采用与薄层检测方法相同的提取方法，除了2%至3%的EMIT - d.a.u.阳性结果外，可为所有检测方法提供确证。
