Abercrombie M L, Jewell J S
J Anal Toxicol. 1986 Sep-Oct;10(5):178-80. doi: 10.1093/jat/10.5.178.
Results of EMIT, Abuscreen RIA, and GC/MS tests for THC metabolites in a high volume random urinalysis program are compared. Samples were field tested by non-laboratory personnel with an EMIT system using a 100 ng/mL cutoff. Samples were then sent to the Army Forensic Toxicology Drug Testing Laboratory (WRAMC) at Fort Meade, Maryland, where they were tested by RIA (Abuscreen) using a statistical 100 ng/mL cutoff. Confirmations of all RIA positives were accomplished using a GC/MS procedure. EMIT and RIA results agreed for 91% of samples. Data indicated a 4% false positive rate and a 10% false negative rate for EMIT field testing. In a related study, results for samples which tested positive by RIA for THC metabolites using a statistical 100 ng/mL cutoff were compared with results by GC/MS utilizing a 20 ng/mL cutoff for the THCA metabolite. Presence of THCA metabolite was detected in 99.7% of RIA positive samples. No relationship between quantitations determined by the two tests was found.
比较了在一个大容量随机尿液分析项目中，针对四氢大麻酚代谢物的酶倍增免疫测定法（EMIT）、放射免疫分析（Abuscreen RIA）和气相色谱/质谱联用（GC/MS）测试的结果。样本由非实验室人员使用EMIT系统进行现场检测，临界值设定为100纳克/毫升。然后样本被送往位于马里兰州米德堡的陆军法医毒理学药物检测实验室（WRAMC），在那里使用统计学临界值100纳克/毫升通过放射免疫分析（Abuscreen）进行检测。所有放射免疫分析阳性结果均通过气相色谱/质谱联用程序进行确认。酶倍增免疫测定法和放射免疫分析结果在91%的样本中一致。数据表明，酶倍增免疫测定法现场检测的假阳性率为4%，假阴性率为10%。在一项相关研究中，将使用统计学临界值100纳克/毫升通过放射免疫分析检测出四氢大麻酚代谢物呈阳性的样本结果，与使用四氢大麻酸（THCA）代谢物临界值20纳克/毫升的气相色谱/质谱联用结果进行了比较。在99.7%的放射免疫分析阳性样本中检测到了四氢大麻酸代谢物。未发现两种检测方法所测定的定量之间存在关联。
