对果蝇头部进行冷冻置换，以便后续进行[3H]2-脱氧葡萄糖放射自显影。

Freeze-substitution of Drosophila heads for subsequent [3H]2-deoxyglucose autoradiography.

作者信息

Rodrigues V, Bülthoff I

出版信息

J Neurosci Methods. 1985 May;13(3-4):183-90. doi: 10.1016/0165-0270(85)90066-4.

Abstract

High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at -76 degrees C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.

摘要

在-76℃无水丙酮中进行冷冻置换后，在果蝇大脑的放射自显影片中获得了[3H]2-脱氧葡萄糖标记的高分辨率图像。该方法应用于接受视觉、嗅觉和机械感觉刺激的标本。将放射自显影片与冷冻干燥后获得的影片进行比较。冷冻置换技术简单、快速且成本低廉，能产生良好的组织保存质量，因此推荐用于放射自显影前的组织脱水。