The deoxyglucose method for insects: towards electron microscopical resolution.

The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.