Gačnikar J, Mrkun J, Babič J, Sterniša M, Zakošek Pipan M
Clinic for Reproduction and Large Animals, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
Institute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
Vet Sci. 2024 Jun 11;11(6):267. doi: 10.3390/vetsci11060267.
The quality of animal feed is increasingly affected by weather conditions, high humidity, and damage to grains, which have led to various mycotoxin-producing moulds. The aim of this study was to determine the effects of the combination of deepoxy-deoxynivalenol and beta-zearalenol on the development of preimplantation bovine embryos, the extent to which the presence of both mycotoxin metabolites affects the development of in vitro cultured bovine embryos, or whether the effect of both toxins enhances embryotoxicity. Ovaries were transported from the abattoir to the laboratory and, after maturation and fertilisation, zygotes were placed in an embryo culture medium (IVC) with different mycotoxin metabolite concentrations diluted in acetonitrile. It was found that the blastocyst rate of cleaved embryos was affected by 1 μL acetonitrile in 400 μL medium (0.25%) compared to the group without acetonitrile. For this reason, it was decided to use acetonitrile as a control group, and the desired mycotoxin metabolite concentrations were diluted in the lowest possible amount of acetonitrile (0.5 μL) that could be accurately added to the study groups. There was no statistical difference when the higher mycotoxin metabolite concentrations were added.
动物饲料的质量越来越受到天气条件、高湿度和谷物受损的影响，这些因素导致了各种产生霉菌毒素的霉菌。本研究的目的是确定去环氧脱氧雪腐镰刀菌烯醇和β-玉米赤霉烯醇的组合对植入前牛胚胎发育的影响，这两种霉菌毒素代谢物的存在对体外培养的牛胚胎发育的影响程度，或者这两种毒素的作用是否会增强胚胎毒性。卵巢从屠宰场运至实验室，在成熟和受精后，将受精卵置于含有不同浓度霉菌毒素代谢物（用乙腈稀释）的胚胎培养基（体外培养）中。结果发现，与不含乙腈的组相比，在400μL培养基中加入1μL乙腈（0.25%）会影响分裂胚胎的囊胚率。因此，决定将乙腈用作对照组，并将所需的霉菌毒素代谢物浓度用尽可能低的乙腈量（0.5μL）稀释后准确添加到研究组中。添加较高浓度的霉菌毒素代谢物时没有统计学差异。
