Alm H, Greising T, Brüssow K-P, Torner H, Tiemann U
Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany.
Toxicol In Vitro. 2002 Dec;16(6):643-8. doi: 10.1016/s0887-2333(02)00059-0.
The aim of the present study was to investigate the influence of specific toxins on in vitro maturation and embryo culture. alpha- and beta-zearalenol were tested at increasing levels from 3.75 to 90 microM and deoxynivalenol from 0.94 to 7.5 microM in order to evaluate the effect on in vitro maturation rate of porcine cumulus-oocyte complexes. Furthermore, the influence of alpha-zearalenol (3.75-30 microM) was appraised on the developmental competence of in vivo-derived zygotes during 5 days of in vitro culture. All three substances affected maturation and degeneration rates in a dose-dependent manner, but to different extents. Significant differences were obtained at a concentration of 7.5 microM alpha-zearalenol and higher. beta-zearalenol negatively affected the process of oocyte development beginning at a concentration of 30.0 microM (P<0.05). Deoxynivalenol had significant influence on oocyte maturation at a concentration of 1.88 microM (31.4 vs 79.3% for control). Differences in embryonic development in vitro were observed at a concentration of 15 microM alpha-zearalenol (P<0.05). These data demonstrate a negative effect of alpha-zearalenol on embryonic development of zygotes, and a compound-specific, dose-dependent negative effect of the three substances on meiotic progression of porcine oocytes.
本研究的目的是调查特定毒素对体外成熟和胚胎培养的影响。测试了α-玉米赤霉烯醇和β-玉米赤霉烯醇，浓度从3.75微摩尔升至90微摩尔，脱氧雪腐镰刀菌烯醇浓度从0.94微摩尔升至7.5微摩尔，以评估对猪卵丘-卵母细胞复合体体外成熟率的影响。此外，评估了α-玉米赤霉烯醇（3.75 - 30微摩尔）对体内来源合子在体外培养5天期间发育能力的影响。所有三种物质均以剂量依赖方式影响成熟和退化率，但程度不同。在α-玉米赤霉烯醇浓度为7.5微摩尔及更高时获得显著差异。β-玉米赤霉烯醇从浓度30.0微摩尔开始对卵母细胞发育过程产生负面影响（P<0.05）。脱氧雪腐镰刀菌烯醇在浓度1.88微摩尔时对卵母细胞成熟有显著影响（对照组为79.3%，处理组为31.4%）。在α-玉米赤霉烯醇浓度为15微摩尔时观察到体外胚胎发育存在差异（P<0.05）。这些数据表明α-玉米赤霉烯醇对合子胚胎发育有负面影响，并且这三种物质对猪卵母细胞减数分裂进程有化合物特异性、剂量依赖性的负面影响。
