College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China; Ley Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.
College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China.
Talanta. 2024 Oct 1;278:126402. doi: 10.1016/j.talanta.2024.126402. Epub 2024 Jun 24.
Simultaneous detection of multiple biomarker levels is essential to improve the accuracy of early diagnosis. Introducing capillary will simplify procedure, less time, and reduce reagent consumption for point-of-care testing of biomarkers. Here, we developed a portable and controllable smartphone-integrated fluorescence capillary imprinted sensing platform for the accuracy visual detection of Crohn's disease biomarkers (lysozyme, Fe) using single-excitation/double-signal detection. A novel controllable capillary coating strategy was developed by static gas-driven coating method for synthesis uniform fluorescence capillary imprinted sensor (Si-CD/g-CdTe@MIP capillary sensor). When Fe and lysozyme were added, the fluorescence intensity of Si-CD/g-CdTe@MIP capillary sensor was quenched at 426 nm and enhanced at 546 nm, respectively. This Si-CD/g-CdTe@MIP capillary sensor has high sensitivity and selectivity for quantification lysozyme and Fe simultaneously with the detection limit of 0.098 nM and 0.20 nM, respectively. In addition, the smartphone-integrated Si-CD/g-CdTe@MIP capillary sensor was applied for the intelligent detection of lysozyme and Fe, in which the detection limit was calculated as 0.32 nM and 0.65 nM. The smartphone-integrated visual Si-CD/g-CdTe@MIP capillary sensor realized ultrasensitive microanalysis (18 μL/time) of biomarkers in health man and Crohn 's patients, providing a novel strategy for early diagnosis of Crohn 's disease.
同时检测多种生物标志物水平对于提高早期诊断的准确性至关重要。引入毛细管将简化操作流程，减少检测生物标志物所需的时间和试剂消耗。在这里，我们开发了一种便携式、可控制的智能手机集成荧光毛细管印迹传感平台，用于使用单激发/双信号检测对克罗恩病生物标志物（溶菌酶、Fe）进行精确的可视化检测。通过静态气体驱动涂层方法开发了一种新的可控毛细管涂层策略，用于合成均匀的荧光毛细管印迹传感器（Si-CD/g-CdTe@MIP 毛细管传感器）。当添加 Fe 和溶菌酶时，Si-CD/g-CdTe@MIP 毛细管传感器的荧光强度分别在 426nm 处猝灭和在 546nm 处增强。这种 Si-CD/g-CdTe@MIP 毛细管传感器对溶菌酶和 Fe 的同时定量检测具有高灵敏度和选择性，检测限分别为 0.098nM 和 0.20nM。此外，智能手机集成的 Si-CD/g-CdTe@MIP 毛细管传感器用于智能检测溶菌酶和 Fe，检测限分别计算为 0.32nM 和 0.65nM。智能手机集成的可视化 Si-CD/g-CdTe@MIP 毛细管传感器实现了健康人和克罗恩病患者生物标志物的超灵敏微量分析（每次 18μL），为克罗恩病的早期诊断提供了一种新策略。
