Chen Yu, Tang Kangling, Zhou Qin, Wang Xiangni, Wang Ruoyan, Zhang Zhaohui
College of Biological and Chemical Engineering, Changsha University, Changsha 410022, PR China.
College of Chemistry and Chemical Engineering, Jishou University, Jishou,Hunan 416000, China.
Anal Chem. 2023 Dec 12;95(49):18139-18148. doi: 10.1021/acs.analchem.3c03571. Epub 2023 Nov 28.
Herein, a dual-nanozyme cascade catalysis triemission fluorescence capillary imprinted sensor integrated with intelligent logic gates was constructed for simultaneous detection of 2,4-dichlorophenoxyacetic acid (2,4-DA) and 2,4-dichlorophenol (2,4-DCP). The novel nanozyme fluorescence organic framework (Bi, Co-MOF) was grafted on the surface of FeO modified with histidine to form a nanozyme composite (FBM) with dual-enzyme activity, which was imprinted with 2,4-DA to prepare a fluorescence molecularly imprinted polymer (FBM@MIP). Carbon dots (CDs) coupling with FBM@MIP (FBM@MIP/CDs) was inhaled into a capillary to construct a dual-nanozyme capillary imprinted sensor directly. The FBM@MIP/CDs capillary sensor realized to detect 2,4-DA and 2,4-DCP simultaneously within a linear concentration range of 1.0 × 10-1.2 × 10 M and 1.0 × 10-4.8 × 10 M with the detection limit of 0.75 and 0.68 pM, respectively. Interestingly, a smartphone-assisted portable capillary fluorescence intelligent sensing platform was developed that can detect 2,4-DA and 2,4-DCP visually without tedious operations such as soaking and drying. Combined with a smartphone, the linear relationships between RGB ratios and concentrations of 2,4-DA and 2,4-DCP were established with the detection limit of 0.93 and 0.81 pM, respectively. The integrated logic gates provided a promising way for intelligent sensing of multiple targets simultaneously, which provided a new strategy for ultrasensitive simultaneous detection of multiple pollutants with a microvolume (18 μL/time) in complex environments.
在此，构建了一种集成智能逻辑门的双纳米酶级联催化三发射荧光毛细管印迹传感器，用于同时检测2,4-二氯苯氧乙酸（2,4-DA）和2,4-二氯苯酚（2,4-DCP）。将新型纳米酶荧光有机框架（Bi, Co-MOF）接枝到用组氨酸修饰的FeO表面，形成具有双酶活性的纳米酶复合材料（FBM），用2,4-DA进行印迹以制备荧光分子印迹聚合物（FBM@MIP）。将与FBM@MIP偶联的碳点（CDs）（FBM@MIP/CDs）吸入毛细管中，直接构建双纳米酶毛细管印迹传感器。FBM@MIP/CDs毛细管传感器实现了在1.0×10⁻¹.²×10 M和1.0×10⁻⁴.⁸×10 M的线性浓度范围内同时检测2,4-DA和2,4-DCP，检测限分别为0.75和0.68 pM。有趣的是，开发了一种智能手机辅助的便携式毛细管荧光智能传感平台，该平台无需浸泡和干燥等繁琐操作即可直观地检测2,4-DA和2,4-DCP。结合智能手机，建立了RGB比值与2,4-DA和2,4-DCP浓度之间的线性关系，检测限分别为0.93和0.81 pM。集成逻辑门为同时智能传感多个目标提供了一种有前景的方法，为在复杂环境中以微体积（每次18 μL）超灵敏同时检测多种污染物提供了一种新策略。
