Okinawa Marine Research Center, Okinawa, Japan.
Laboratory of Veterinary Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan.
J Vet Med Sci. 2024 Aug 2;86(8):872-876. doi: 10.1292/jvms.23-0523. Epub 2024 Jun 27.
In this study, we examined the usefulness of a simpler and more feasible method for determining the optimal timing of artificial insemination and the conditions for its success in six female common bottlenose dolphins. Pregnancy was successfully achieved in five dolphins by performing intrauterine insemination, using chilled semen stored for less than 3 days or frozen semen within 24 hr of exhibiting a peak serum estradiol (E) level of 100 pg/mL or higher or on the day with a serum E level of approximately 100 pg/mL, measured with a simple measuring device. We concluded that the determining the optimal timing of intrauterine insemination by measuring serum E levels is a simpler and more useful method compared with the conventional approach.
在这项研究中，我们检查了一种更简单、更可行的方法在六头雌性宽吻海豚中确定人工授精最佳时间和成功条件的有用性。通过使用冷藏精液（储存时间少于 3 天）或在血清雌二醇（E）水平达到 100pg/ml 峰值后 24 小时内或在使用简单测量设备测量血清 E 水平约为 100pg/ml 的当天进行宫内授精，成功地使五头海豚怀孕。我们得出结论，与传统方法相比，通过测量血清 E 水平来确定宫内授精的最佳时间是一种更简单、更有用的方法。
