• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

建立和应用一个新型的蒙古沙鼠 SNP 遗传检测面板。

Establishment and Application of a Novel Genetic Detection Panel for SNPs in Mongolian Gerbils.

机构信息

School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Laboratory for Clinical Medicine, Capital Medical University, Beijing 100069, China.

出版信息

Genes (Basel). 2024 Jun 20;15(6):817. doi: 10.3390/genes15060817.

DOI:10.3390/genes15060817
PMID:38927752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11202554/
Abstract

The Mongolian gerbil is a distinctive experimental animal in China, as its genetic qualities possess significant value in the field of medical biology research. Here, we aimed to establish an economical and efficient panel for genetic quality detection in Mongolian gerbils using single-nucleotide polymorphism (SNP) markers. To search for SNPs, we conducted whole-genome sequencing (WGS) in 40 Mongolian gerbils from outbred populations. Reliable screening criteria were established to preliminarily select SNPs with a wide genome distribution and high levels of polymorphism. Subsequently, a multiple-target regional capture detection system based on second-generation sequencing was developed for SNP genotyping. Based on the results of WGS, 219 SNPs were preliminarily selected, and they were established and optimized in a multiple-amplification system that included 206 SNP loci by genotyping three outbred populations. PopGen.32 analysis revealed that the average effective allele number, Shannon index, observed heterozygosity, expected heterozygosity, average heterozygosity, polymorphism information content, and other population genetic parameters of the Capital Medical University (CMU) gerbils were the highest, followed by those of Zhejiang gerbils and Dalian gerbils. Through scientific screening and optimization, we successfully established a novel, robust, and cost-effective genetic detection system for Mongolian gerbils by utilizing SNP markers for the first time.

摘要

蒙古沙鼠是中国特有的实验动物，其遗传品质在医学生物学研究领域具有重要价值。在这里，我们旨在使用单核苷酸多态性 (SNP) 标记建立蒙古沙鼠遗传质量检测的经济高效面板。为了寻找 SNP，我们对来自远交种群的 40 只蒙古沙鼠进行了全基因组测序 (WGS)。建立了可靠的筛选标准，初步选择了具有广泛基因组分布和高水平多态性的 SNP。随后，基于第二代测序开发了一种基于多靶区域捕获检测的 SNP 基因分型系统。基于 WGS 的结果，初步选择了 219 个 SNP，并通过对三个远交种群的基因分型，在包括 206 个 SNP 位点的多重扩增系统中建立和优化了这些 SNP。PopGen.32 分析表明，首都医科大学（CMU）沙鼠的平均有效等位基因数、香农指数、观察杂合度、期望杂合度、平均杂合度、多态信息含量等群体遗传参数最高，其次是浙江沙鼠和大连沙鼠。通过科学筛选和优化，我们首次利用 SNP 标记成功建立了一种新型、稳健、经济高效的蒙古沙鼠遗传检测系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/2a9631559b31/genes-15-00817-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/f3767748a3b1/genes-15-00817-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/3d4b8c69c130/genes-15-00817-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/ad6862e08253/genes-15-00817-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/2a9631559b31/genes-15-00817-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/f3767748a3b1/genes-15-00817-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/3d4b8c69c130/genes-15-00817-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/ad6862e08253/genes-15-00817-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f6/11202554/2a9631559b31/genes-15-00817-g004.jpg

相似文献

1
Establishment and Application of a Novel Genetic Detection Panel for SNPs in Mongolian Gerbils.建立和应用一个新型的蒙古沙鼠 SNP 遗传检测面板。
Genes (Basel). 2024 Jun 20;15(6):817. doi: 10.3390/genes15060817.
2
Development of novel microsatellite DNA markers by cross-amplification and analysis of genetic variation in gerbils.通过交叉扩增和遗传变异分析开发沙鼠新型微卫星 DNA 标记。
J Hered. 2010 Nov-Dec;101(6):710-6. doi: 10.1093/jhered/esq066. Epub 2010 Jun 4.
3
Comparison of whole-genome (13X) and capture (87X) resequencing methods for SNP and genotype callings.全基因组（13X）和捕获（87X）重测序方法在单核苷酸多态性（SNP）和基因型检测方面的比较。
Anim Genet. 2015 Feb;46(1):82-6. doi: 10.1111/age.12248. Epub 2014 Dec 16.
4
[Genetic diversity of Mongolian gerbils (Meriones unguiculatus)].[长爪沙鼠（Meriones unguiculatus）的遗传多样性]
Yi Chuan. 2008 Jul;30(7):877-84. doi: 10.3724/sp.j.1005.2008.00877.
5
A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties.一种新的 SNP 基因分型技术——靶向 SNP-seq 及其在黄瓜品种遗传分析中的应用。
Sci Rep. 2020 Mar 27;10(1):5623. doi: 10.1038/s41598-020-62518-6.
6
Variant calling and genotyping accuracy of ddRAD-seq: Comparison with 20X WGS in layers.ddRAD-seq 变异调用和基因分型准确性：与 20X WGS 在层中的比较。
PLoS One. 2024 Jul 26;19(7):e0298565. doi: 10.1371/journal.pone.0298565. eCollection 2024.
7
An evaluation of sequencing coverage and genotyping strategies to assess neutral and adaptive diversity.评估测序覆盖度和基因分型策略，以评估中性和适应性多样性。
Mol Ecol Resour. 2019 Nov;19(6):1497-1515. doi: 10.1111/1755-0998.13070. Epub 2019 Sep 9.
8
Genetic polymorphism and population structure of Torghut Mongols and comparison with a Mongolian population 3000 kilometers away.托和特蒙古族的遗传多态性和种群结构与 3000 公里外的蒙古族人群进行比较。
Forensic Sci Int Genet. 2019 Sep;42:235-243. doi: 10.1016/j.fsigen.2019.07.017. Epub 2019 Jul 29.
9
Genetic consequences of group living in Mongolian gerbils.群体生活对蒙古沙鼠的遗传影响。
J Hered. 2011 Sep-Oct;102(5):554-61. doi: 10.1093/jhered/esr069. Epub 2011 Jul 21.
10
Targeted capture and sequencing of 1245 SNPs for forensic applications.针对法医应用的 1245 个 SNP 的靶向捕获和测序。
Forensic Sci Int Genet. 2019 Sep;42:227-234. doi: 10.1016/j.fsigen.2019.07.006. Epub 2019 Jul 15.

引用本文的文献

1
Development of a single-nucleotide polymorphism panel genotyping system for genetic analysis of Chinese hamsters.用于中国仓鼠遗传分析的单核苷酸多态性面板基因分型系统的开发。
Animal Model Exp Med. 2025 May;8(5):916-921. doi: 10.1002/ame2.70006. Epub 2025 Feb 27.

本文引用的文献

1
Study on the Difference of Protective Efficacy and Mechanism of Radix Aconiti Coreani and Rhizoma Typhonii in Gerbils with Ischemic Stroke.红乌头和南星对沙土鼠缺血性脑卒中保护作用及机制的差异研究。
ACS Chem Neurosci. 2023 Oct 4;14(19):3686-3693. doi: 10.1021/acschemneuro.3c00299. Epub 2023 Sep 12.
2
An MPS-Based 50plex Microhaplotype Assay for Forensic DNA Analysis.基于 MPS 的 50 重微单倍型检测法在法医 DNA 分析中的应用。
Genes (Basel). 2023 Apr 4;14(4):865. doi: 10.3390/genes14040865.
3
Identification and functional assessment of 10 CYP2C9 variants found in Chinese Han subjects.
鉴定和功能评估在中国汉族人群中发现的 10 种 CYP2C9 变异体。
Front Endocrinol (Lausanne). 2023 Mar 15;14:1139805. doi: 10.3389/fendo.2023.1139805. eCollection 2023.
4
Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage.全外显子组测序在不明原因复发性流产家系中鉴定出了非整倍体流产的新的致病性遗传病因。
Hum Reprod. 2023 May 2;38(5):1003-1018. doi: 10.1093/humrep/dead039.
5
Neuroprotective Effects of Ethanol Extract of (EEPF) against Glutamate-Mediated Neuronal Toxicity in HT22 Cells.（EEPF）乙醇提取物对 HT22 细胞谷氨酸介导的神经毒性的神经保护作用。
Int J Mol Sci. 2023 Feb 16;24(4):3969. doi: 10.3390/ijms24043969.
6
Genome-wide association study finds multiple loci associated with intraocular pressure in HS rats.全基因组关联研究发现多个与HS大鼠眼压相关的基因座。
Front Genet. 2023 Jan 30;13:1029058. doi: 10.3389/fgene.2022.1029058. eCollection 2022.
7
A comprehensive evaluation of an animal model for Helicobacter pylori-associated stomach cancer: Fact and controversy.幽门螺杆菌相关性胃癌动物模型的综合评价：事实与争议
Helicobacter. 2023 Feb;28(1):e12943. doi: 10.1111/hel.12943. Epub 2023 Jan 10.
8
Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil.给研究人员的信息：在后交通动脉缺失的特征在沙鼠中很容易丢失。
Anat Sci Int. 2023 Jul;98(3):426-433. doi: 10.1007/s12565-022-00698-z. Epub 2022 Dec 6.
9
A germline SNP in BRMS1 predisposes patients with lung adenocarcinoma to metastasis and can be ameliorated by targeting c-fos.BRMS1 中的种系 SNP 使肺腺癌患者易发生转移，并可通过靶向 c-fos 加以改善。
Sci Transl Med. 2022 Oct 5;14(665):eabo1050. doi: 10.1126/scitranslmed.abo1050.
10
A common SNP in Chrna5 enhances morphine reward in female mice.Chrna5基因上的一个常见单核苷酸多态性增强了雌性小鼠对吗啡的奖赏效应。
Neuropharmacology. 2022 Nov 1;218:109218. doi: 10.1016/j.neuropharm.2022.109218. Epub 2022 Aug 13.