State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Int J Mol Sci. 2024 Jun 18;25(12):6694. doi: 10.3390/ijms25126694.
Phytochemicals and tryptophan (Trp) metabolites have been found to modulate gut function and health. However, whether these metabolites modulate gut ion transport and serotonin (5-HT) metabolism and signaling requires further investigation. The aim of this study was to investigate the effects of selected phytochemicals and Trp metabolites on the ion transport and 5-HT metabolism and signaling in the ileum of mice in vitro using the Ussing chamber technique. During the in vitro incubation, vanillylmandelic acid (VMA) reduced ( < 0.05) the short-circuit current, and 100 μM chlorogenic acid (CGA) ( = 0.12) and perillic acid (PA) ( = 0.14) had a tendency to reduce the short-circuit current of the ileum. Compared with the control, PA and -acetylserotonin treatment upregulated the expression of tryptophan hydroxylase 1 (), while 100 μM cinnamic acid, indolelactic acid (ILA), and 10 μM CGA or indoleacetaldehyde (IAld) treatments downregulated ( < 0.05) the mRNA levels of . In addition, 10 μM IAld or 100 μM ILA upregulated ( < 0.05) the expression of monoamine oxidase A (). However, 10 μM CGA or 100 μM PA downregulated ( < 0.05) expression. All selected phytochemicals and Trp metabolites upregulated ( < 0.05) the expression of and compared to that of the control group. VMA and CGA reduced ( < 0.05) the ratios of / and /. These findings may help to elucidate the effects of phytochemicals and Trp metabolites on the regulation of gut ion transport and 5-HT signaling-related gut homeostasis in health and disease.
植物化学物质和色氨酸（Trp）代谢物已被发现可调节肠道功能和健康。然而，这些代谢物是否调节肠道离子转运和 5-羟色胺（5-HT）代谢和信号传递仍需要进一步研究。本研究旨在使用 Ussing 室技术，在体外研究选定的植物化学物质和 Trp 代谢物对小鼠回肠离子转运和 5-HT 代谢和信号传递的影响。在体外孵育期间，香草扁桃酸（VMA）降低（<0.05）短路电流，而 100 μM 绿原酸（CGA）（=0.12）和紫苏酸（PA）（=0.14）有降低回肠短路电流的趋势。与对照组相比，PA 和 -乙酰色氨酸处理上调了色氨酸羟化酶 1（）的表达，而 100 μM 肉桂酸、吲哚乳酸（ILA）和 10 μM CGA 或吲哚乙醛（IAld）处理下调了（<0.05）的 mRNA 水平。此外，10 μM IAld 或 100 μM ILA 上调（<0.05）单胺氧化酶 A（）的表达。然而，10 μM CGA 或 100 μM PA 下调（<0.05）的表达。与对照组相比，所有选定的植物化学物质和 Trp 代谢物均上调（<0.05）的表达。VMA 和 CGA 降低（<0.05）/和/的比值。这些发现可能有助于阐明植物化学物质和 Trp 代谢物对肠道离子转运和 5-HT 信号相关肠道内稳态在健康和疾病中的调节作用。
