Jose-Salazar Jorge Alberto, Ballinas-Cesatti Christian Bryan, Hernández-Martínez Diana Maylet, Cristiani-Urbina Eliseo, Melgar-Lalanne Guiomar, Morales-Barrera Liliana
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional Adolfo López Mateos, Ciudad de México 07738, Mexico.
Departamento de Biofísica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Ciudad de México 11340, Mexico.
Foods. 2024 Jun 20;13(12):1948. doi: 10.3390/foods13121948.
There is a growing market for craft beverages with unique flavors. This study aimed to obtain a palate-pleasing mead derived from 4A as a monoculture. Different culture media were evaluated to compare the fermentation kinetics and final products. The crucial factors in the medium were 200 mg L of yeast assimilable nitrogen and a pH of 3.5-5.0. A panel of judges favored the mead derived from 4A (fermented in a medium with honey initially at 23 °Bx) over a commercial sample produced from , considering its appearance, fruity and floral flavors (provided by esters, aldehydes, and higher alcohols), and balance between sweetness (given by the 82.91 g L of residual sugars) and alcohol. The present mead had an 8.57% / ethanol concentration, was elaborated in 28 days, and reached a maximum biomass growth (2.40 g L) on the same fermentation day (6) that the minimum level of pH was reached. The biomass growth yield peaked at 24 and 48 h (0.049 g g), while the ethanol yield peaked at 24 h (1.525 ± 0.332 g g), in both cases declining thereafter. The Gompertz model adequately describes the kinetics of sugar consumption and the generation of yeast biomass and ethanol. Pathogenic microorganisms, methanol, lead, and arsenic were absent in the mead. Thus, 4A produced a safe and quality mead with probable consumer acceptance.
具有独特风味的精酿饮料市场正在不断扩大。本研究旨在获得一种以4A作为单一培养物的口感宜人的蜂蜜酒。对不同的培养基进行了评估,以比较发酵动力学和最终产品。培养基中的关键因素是约200 mg/L的酵母可同化氮和pH值3.5 - 5.0。一组评委认为,与市售样品相比,由4A(在初始糖度为23°Bx的蜂蜜培养基中发酵)制成的蜂蜜酒在外观、水果和花香风味(由酯类、醛类和高级醇提供)以及甜度(由82.91 g/L的残余糖分提供)与酒精之间的平衡方面更具优势。目前的蜂蜜酒乙醇浓度为8.57%,酿造时间为28天,在达到最低pH值的同一天(第6天)达到最大生物量增长(2.40 g/L)。生物量增长产量在24小时和48小时达到峰值(约0.049 g/g),而乙醇产量在24小时达到峰值(1.525±0.332 g/g),此后两者均下降。Gompertz模型充分描述了糖消耗、酵母生物量和乙醇生成的动力学。蜂蜜酒中不存在致病微生物、甲醇、铅和砷。因此,4A生产出了一种安全且质量上乘、可能会被消费者接受的蜂蜜酒。