Taketani S, Nishino T, Katsuki H
Biochim Biophys Acta. 1979 Oct 26;575(1):148-55.
Cell-free extracts of Saccharomyces cerevisiae grown under aerobic as well as semi-anaerobic conditions were found to catalyze the synthesis of fatty acid ester of sterol from cholesterol, fatty acid, ATP and CoA, or from cholesterol and fatty acyl-CoA. This result indicates that the enzyme involved in the formation of the ester is acyl-CoA:sterol O-acyltransferase (EC 2.3.1.26). The enzyme had a broad substrate specificity for sterols and acyl-CoAs. The enzyme levels in the cells grown under aerobic and semi-anaerobic conditions were almost equal. The enzyme was located in the microsomal fraction of the aerobically grown cells.
研究发现，在有氧及半厌氧条件下生长的酿酒酵母无细胞提取物能够催化由胆固醇、脂肪酸、ATP和辅酶A，或由胆固醇和脂肪酰辅酶A合成甾醇脂肪酸酯。该结果表明，参与酯形成的酶是酰基辅酶A：甾醇O-酰基转移酶（EC 2.3.1.26）。该酶对甾醇和酰基辅酶A具有广泛的底物特异性。在有氧和半厌氧条件下生长的细胞中，该酶的水平几乎相等。该酶位于有氧生长细胞的微粒体部分。
