Saathoff M, Seitz H M
Z Geburtshilfe Perinatol. 1985 Mar-Apr;189(2):73-8.
Serum samples from 702 persons were examined for Toxoplasma-specific IgM-antibodies using the immunosorbent agglutination assay (ISAGA) and the immunofluorescence-test (IIFT). 250 samples showed a positive reaction in the Sabin-Feldman-test (SFT) with titers greater than or equal to 1: 1 024, 58% were positive in ISAGA and 36% in the IIFT. Samples of persons with acute Toxoplasma-infection, showed high titers in the ISAGA and SFT as well, even in cases where the IgM-IIFT was negative. SFT-negative sera and others with weakly-positive reactions and also rheuma-positive samples were negative in the ISAGA. It is discussed how far it is possible to determine the duration of the Toxoplasma infection by applying the ISAGA in combination with other antibody tests.
采用免疫吸附凝集试验（ISAGA）和免疫荧光试验（IIFT）对702人的血清样本进行了弓形虫特异性IgM抗体检测。250份样本在Sabin-Feldman试验（SFT）中呈阳性反应，滴度大于或等于1:1024，ISAGA检测阳性率为58%，IIFT检测阳性率为36%。急性弓形虫感染患者的样本在ISAGA和SFT中也显示出高滴度，即使在IgM-IIFT检测为阴性的情况下也是如此。SFT阴性血清以及其他弱阳性反应血清和类风湿阳性样本在ISAGA检测中均为阴性。本文讨论了联合应用ISAGA和其他抗体检测方法来确定弓形虫感染持续时间的可行性。
