[Testing of a modified Toxoplasma immunosorbent-adsorption assay (IgM-ISAGA) for the detection of Toxoplasma-specific IgM antibodies in the routine laboratory].

Testing of a modified Toxoplasma immunosorbent adsorption assay (IgM-ISAGA) for proving of Toxoplasma specific IgM antibodies. The IgM-ISAGA was assessed for the possibility of its application as a routine method in the serodiagnosis of toxoplasmosis and was compared with the IgM-IFT. On examination for specific on 136 sera, no false-positive results were obtained in the IgM-ISAGA in contrast to 5 false-positives in the IgM-IFT, due to the presence of rheumatoid factor. Its sensitivity was tested on 162 sera. The IgM-ISAGA reacted positive in 89 cases, the IgM-IFT in only 51. It was concluded that the IgM-ISAGA is a simple, sensitive and highly specific test applicable in smaller laboratories as a confirmatory reaction in the control of toxoplasmosis during pregnancy and in confirmation of connatal toxoplasmosis.