Department of Inorganic Chemistry, Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387 Kraków, Poland.
Doctoral School of Exact and Natural Sciences, Jagiellonian University, Prof. St. Łojasiewicza St 11, 30-348 Kraków, Poland.
Molecules. 2024 Jun 8;29(12):2735. doi: 10.3390/molecules29122735.
The growth of , a member of the genus, which plays a crucial role in the bacterial microbiome of the gut, is significantly influenced by manganese ions. They can be safely delivered to the intestines by exploiting the chelating abilities of lactoferrin. The aim of this work was to encapsulate lactoferrin saturated with manganese ions (MnLf) in a system based on the Eudragit RS polymer to protect protein from degradation and manganese release in the gastric environment. The entrapment efficiency was satisfactory, reaching about 95%, and most importantly, manganese ions were not released during microparticles (MPs) formation. The release profile of the protein from the freshly prepared MPs was sustained, with less than 15% of the protein released within the first hour. To achieve similar protein release efficiency, freeze-drying was carried out in the presence of 10% (/) mannitol as a cryoprotectant for MPs frozen at -20 °C. MPs with encapsulated MnLf exhibited prebiotic activity towards . More importantly, the presence of equivalent levels of manganese ions in free form in the medium, as well as chelating by lactoferrin encapsulated in MPs, had a similar impact on stimulating bacterial growth. This indicates that the bioavailability of manganese ions in our prepared system is very good.
肠内菌群中 属的一员 的生长受到锰离子的显著影响。乳铁蛋白利用其螯合能力，可以将锰离子安全地递送到肠道。本工作旨在将饱和锰离子的乳铁蛋白（MnLf）包封在基于 Eudragit RS 聚合物的系统中，以保护蛋白质免受胃环境中降解和锰释放的影响。包封效率令人满意，达到约 95%，更重要的是，在微球（MPs）形成过程中没有释放出锰离子。从新制备的 MPs 中释放蛋白质的释放曲线是持续的，在第一个小时内释放的蛋白质少于 15%。为了达到类似的蛋白质释放效率，在存在 10%（/）甘露醇作为保护剂的情况下进行冷冻干燥，将 MPs 在-20°C 下冷冻。包封 MnLf 的 MPs 对 表现出益生元活性。更重要的是，在培养基中以游离形式存在的同等水平的锰离子，以及包封在 MPs 中的乳铁蛋白的螯合作用，对刺激细菌生长具有相似的影响。这表明我们制备的系统中锰离子的生物利用度非常好。
