Yeerong Kankanit, Chantawannakul Panuwan, Anuchapreeda Songyot, Juntrapirom Saranya, Kanjanakawinkul Watchara, Müllertz Anette, Rades Thomas, Chaiyana Wantida
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Bee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Pharmaceutics. 2024 May 28;16(6):724. doi: 10.3390/pharmaceutics16060724.
This study aimed to develop chitosan alginate nanoparticles (CANPs) for enhanced stability for dermal delivery of protein hydrolysate from (PH). CANPs, developed using ionotropic pre-gelation followed by the polyelectrolyte complex technique, were characterized for particle size, polydispersity index (PDI), and zeta potential. After the incorporation of PH into CANPs, a comprehensive assessment included encapsulation efficiency, loading capacity, morphology, chemical analyses, physical and chemical stability, irritation potential, release profile, skin permeation, and skin retention. The most optimal CANPs, comprising 0.6 mg/mL sodium alginate, 1.8 mg/mL calcium chloride, and 0.1 mg/mL chitosan, exhibited the smallest particle size (309 ± 0 nm), the narrowest PDI (0.39 ± 0.01), and pronounced negative zeta potential (-26.0 ± 0.9 mV), along with an encapsulation efficiency of 56 ± 2%, loading capacity of 2.4 ± 0.1%, release of 40 ± 2% after 48 h, and the highest skin retention of 12 ± 1%. The CANPs induced no irritation and effectively enhanced the stability of PH from 44 ± 5% of PH remaining in a solution to 74 ± 4% after three-month storage. Therefore, the findings revealed the considerable potential of CANPs in improving PH stability and skin delivery, with promising applications in cosmetics and related fields.
本研究旨在开发壳聚糖海藻酸钠纳米颗粒（CANPs），以提高来自[具体来源未提及]的蛋白质水解物（PH）经皮递送的稳定性。采用离子型预凝胶化随后的聚电解质复合技术制备的CANPs，对其粒径、多分散指数（PDI）和zeta电位进行了表征。将PH包封于CANPs后，进行了全面评估，包括包封效率、载药量、形态、化学分析、物理和化学稳定性、刺激性、释放曲线、皮肤渗透和皮肤滞留。最优化的CANPs由0.6 mg/mL海藻酸钠、1.8 mg/mL氯化钙和0.1 mg/mL壳聚糖组成，其粒径最小（309±0 nm），PDI最窄（0.39±0.01），zeta电位呈显著负值（-26.0±0.9 mV），包封效率为56±2%，载药量为2.4±0.1%，48小时后释放率为40±2%，皮肤滞留率最高为12±1%。CANPs无刺激性，有效提高了PH的稳定性，从溶液中三个月后剩余44±5%的PH提高到74±4%。因此，研究结果揭示了CANPs在提高PH稳定性和经皮递送方面具有巨大潜力，在化妆品及相关领域具有广阔应用前景。
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