Radenković S, Golubović M Velojić, Dimić D, Radojković D B, Ćirić V, Gluvić Z, Bjekić-Macut J, Marković A, Radić L, Pešić M
University Clinical Centre Niš - Clinic for Endocrinology, Diabetes and Metabolic Diseases.
University of Niš, Faculty of Medicine, Niš.
Acta Endocrinol (Buchar). 2023 Oct-Dec;19(4):463-470. doi: 10.4183/aeb.2023.463. Epub 2024 Jun 24.
There are evidences that excessive production of reactive oxygen species is one of important abnormalities that contribute to development of chronic diabetic complications.
To test the effect of intensive insulin therapy with analogues through the examining the level of oxidative stress parameters.
Comparison of data obtained by prospective analysis in 49 patients with T1DM was used, before and after six months of intensive insulin analog therapy.
The values of all three investigated parameters of oxidative stress malondialdehyde (MDA); xanthine oxidase (XO) and nitrates and nitrites (NOx) in our population with T1DM compared to the control (group of 42 voluntary blood donors) are statistically higher. The levels of antioxidant protection parameters compared to the control group also differ; the activities of catalase and glutathione peroxidase (GPx) are statistically higher in our population of T1DM patients compared to the control and superoxide dismutase (SOD) activities are statistically lower.The values of all three examined parameters of oxidative stress decrease after six months of intensive insulin analog therapy and were statistically lower after the therapy: for MDA p<0.001, for XO p<0.01 and for NOx p<0.05. The activities of catalase (p<0.001) and GPx (p<0.01) both decrease with therapy, while the activity of SOD is highest after the sixth month of therapy (p<0.001).
In our patients with T1DM compared to the control the level of oxidative stress is significantly higher. Intensive insulin analog therapy with aspart and glargine promotes predominantly the improvement of oxidative stress, and in a less degree antioxidant protection.
有证据表明活性氧的过量产生是导致慢性糖尿病并发症的重要异常情况之一。
通过检测氧化应激参数水平来测试强化胰岛素类似物治疗的效果。
采用前瞻性分析比较49例1型糖尿病患者在强化胰岛素类似物治疗6个月前后获得的数据。
与对照组（42名自愿献血者）相比，我们的1型糖尿病患者群体中氧化应激的所有三个研究参数丙二醛（MDA）、黄嘌呤氧化酶（XO）以及硝酸盐和亚硝酸盐（NOx）的值在统计学上更高。与对照组相比，抗氧化保护参数水平也有所不同；1型糖尿病患者群体中过氧化氢酶和谷胱甘肽过氧化物酶（GPx）的活性与对照组相比在统计学上更高，而超氧化物歧化酶（SOD）的活性在统计学上更低。强化胰岛素类似物治疗6个月后，氧化应激的所有三个检测参数的值均下降，且治疗后在统计学上更低：MDA的p<0.001，XO的p<0.01，NOx的p<0.05。过氧化氢酶（p<0.001）和GPx（p<0.01）的活性均随治疗而降低，而SOD的活性在治疗6个月后最高（p<0.001）。
与对照组相比，我们的1型糖尿病患者氧化应激水平显著更高。门冬胰岛素和甘精胰岛素强化治疗主要促进氧化应激的改善，对抗氧化保护的促进程度较小。
