Department of Periodontology, Dr. D Y Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India.
Department of Microbiology, Dr. D. Y. Patil Arts Commerce and Science College, Pimpri, Pune, Maharashtra, India.
Indian J Dent Res. 2024 Jan 1;35(1):59-64. doi: 10.4103/ijdr.ijdr_533_23. Epub 2024 Jun 20.
This study aimed to compare the efficacy of subgingivally applied probiotics as an adjunct to scaling and root planing (SRP) vs SRP alone in patients with periodontitis.
Patients diagnosed with periodontitis, with probing pocket depth (PPD) of 5-7 mm on at least two teeth on contralateral sites, were selected for the study and randomly allocated to the test group (n = 31) who underwent SRP along with subgingival application of probiotic paste and the control group (n = 31) who underwent only SRP. Clinical parameters were evaluated in both groups at baseline and after 12 weeks. The viability of probiotic bacteria was evaluated in the test group at baseline, day 4 and day 8.
All clinical parameters showed a statistically significant difference between baseline and 12 weeks on intragroup and intergroup comparison, with a greater improvement in the test group. Microbiological evaluation showed that the mean colony-forming units (CFUs) in the test group were 38.39 ± 7.76, 7.25 ± 2.72 and 1.57 ± 1.29 at baseline, day 4 and day 8, respectively. The mean CFUs significantly reduced with an increase in time from baseline to 8-day time interval.
It was seen that the probiotic bacteria remained viable in the periodontal pocket for up to 8 days after placement, but stable improvements were seen in all clinical parameters even at 12 weeks, indicating its prolonged efficacy. Thus, commercially available probiotics can prove to be an inexpensive method to treat periodontitis when combined with SRP.
本研究旨在比较龈下应用益生菌作为辅助治疗与单独进行牙周洁治（SRP）对牙周炎患者的疗效。
选择患有牙周炎的患者，其在至少两颗对侧部位的牙齿上具有 5-7mm 的探诊袋深度（PPD），入选本研究并随机分配至试验组（n=31），该组患者接受 SRP 联合龈下应用益生菌糊剂，以及对照组（n=31），仅接受 SRP。在基线和 12 周时评估两组的临床参数。在基线、第 4 天和第 8 天评估试验组中益生菌的活菌数。
在组内和组间比较中，所有临床参数均显示基线与 12 周时存在统计学显著差异，试验组的改善更大。微生物学评估显示，试验组的平均菌落形成单位（CFU）在基线、第 4 天和第 8 天分别为 38.39±7.76、7.25±2.72 和 1.57±1.29。CFU 均值随着从基线到 8 天时间间隔的时间增加而显著减少。
可见，益生菌在放置后牙周袋中仍保持存活长达 8 天，但所有临床参数在 12 周时仍显示稳定改善，表明其具有延长的疗效。因此，当与 SRP 联合使用时，市售益生菌可被证明是一种治疗牙周炎的经济有效的方法。
