Gomes-Moura Ana Paula, Araujo Lisa Danielly Curcino, Lucisano Marília Pacífico, Lima Ricardo Barbosa, Magalhães Nilza Letícia, Salvador Sérgio Luiz, Furlaneto Flávia Aparecida Chaves, Messora Michel Reis, Nelson-Filho Paulo, da Silva Raquel Assed Bezerra, da Silva Léa Assed Bezerra
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brasil.
Department of Clinical Analyses, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brasil.
Clin Oral Investig. 2025 May 28;29(6):317. doi: 10.1007/s00784-025-06383-5.
To evaluate the effect of the probiotic Bifidobacterium animalis subsp. lactis (B. lactis) HN019, as an irrigation solution, on the expression of neutrophil extracellular traps (NETs) and the antimicrobial peptide CRAMP, in rats with induced periapical lesions.
Periapical lesions were induced in the first molars of forty-five Wistar Hannover rats, with an experimental period of 21 days. The animals were divided into groups based on the irrigating solution and the number of sessions. Semiquantitative analysis was performed using HE staining, neutrophil counts, and histomorphometric analysis of the periapical lesions. Additionally, immunohistochemistry for CRAMP and immunofluorescence for NETs markers such as H3Cit, MPO, and NE were conducted. qRT-PCR analysis was performed for CAMP, ELANE, MPO, and H3F3B.
The probiotic irrigation groups showed better results for periodontal ligament parameters (p = 0.03) and inflammatory infiltrate (p = 0.004). A single probiotic irrigation resulted in the highest absence of cementum and bone resorption (85.71% and 71.43%). The periapical lesion area was smaller (p = 0.002), and neutrophil counts showed no difference (p = 0.064). Lower immunolabeling for H3Cit (p = 0.002), MPO (p < 0.001), and NE (p = 0.001) was observed in probiotic-irrigated groups, with reduced gene expression of CAMP (p < 0.001), MPO (p = 0.002), and ELANE (p = 0.001).
The probiotic-based irrigation solution was effective in reducing neutrophil extracellular trap formation. Additionally, the probiotic solution stimulated CRAMP expression and had a positive effect in preventing cementum and bone resorption.
The results demonstrate the potential application of the probiotic-based irrigation solution as a promising therapeutic approach for the treatment of periapical lesions.
评估益生菌动物双歧杆菌乳酸亚种（B. lactis）HN019作为冲洗液对诱导性根尖周病变大鼠中性粒细胞胞外诱捕网（NETs）和抗菌肽CRAMP表达的影响。
在45只Wistar Hannover大鼠的第一磨牙上诱导根尖周病变，实验期为21天。根据冲洗液和冲洗次数将动物分组。使用HE染色、中性粒细胞计数和根尖周病变的组织形态计量分析进行半定量分析。此外，进行了CRAMP的免疫组织化学和NETs标志物如H3Cit、MPO和NE的免疫荧光检测。对CAMP、ELANE、MPO和H3F3B进行qRT-PCR分析。
益生菌冲洗组在牙周膜参数（p = 0.03）和炎性浸润（p = 0.004）方面显示出更好的结果。单次益生菌冲洗导致牙骨质和骨吸收的缺失率最高（分别为85.71%和71.43%）。根尖周病变面积较小（p = 0.002），中性粒细胞计数无差异（p = 0.064）。在益生菌冲洗组中观察到H3Cit（p = 0.002）、MPO（p < 0.001）和NE（p = 0.001）的免疫标记较低，CAMP（p < 0.001）、MPO（p = 0.002）和ELANE（p = 0.001）的基因表达降低。
基于益生菌的冲洗液在减少中性粒细胞胞外诱捕网形成方面有效。此外，益生菌溶液刺激了CRAMP表达，并对预防牙骨质和骨吸收有积极作用。
结果表明基于益生菌的冲洗液作为一种有前景的治疗根尖周病变的治疗方法具有潜在应用价值。
