Lauer S J, Browning K S, Ravel J M
Biochemistry. 1985 Jun 4;24(12):2928-31. doi: 10.1021/bi00333a017.
Rabbit polyclonal antibodies to wheat germ initiation factor 3 (eIF-3) were obtained and were shown to react strongly with 4 of the 10 subunits of eIF-3 (pp116, pp87, pp56, and pp36). Two mouse monoclonal antibodies were obtained, one of which reacts specifically with pp87 and one of which reacts specifically with pp36. Highly purified anti-pp87 has no effect on the activity of eIF-3. Highly purified polyclonal antibodies and anti-pp36 inhibit the ability of eIF-3 to support polypeptide synthesis in vitro and the ability of eIF-3 to support mRNA binding to 40S ribosomal subunits. These results provide additional evidence that pp116, pp87, and pp36 are in exposed positions in the eIF-3 particle and that pp36 is essential for activity.
获得了针对小麦胚芽起始因子3（eIF - 3）的兔多克隆抗体，结果显示该抗体与eIF - 3的10个亚基中的4个（pp116、pp87、pp56和pp36）发生强烈反应。获得了两种小鼠单克隆抗体，其中一种特异性地与pp87反应，另一种特异性地与pp36反应。高度纯化的抗pp87对eIF - 3的活性没有影响。高度纯化的多克隆抗体和抗pp36抑制eIF - 3在体外支持多肽合成的能力以及eIF - 3支持mRNA与40S核糖体亚基结合的能力。这些结果提供了额外的证据，表明pp116、pp87和pp36处于eIF - 3颗粒的暴露位置，并且pp36对活性至关重要。
