Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea.
Division of Marine and Fisheries Life Sciences, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2024 Jun 28;34(6):1260-1269. doi: 10.4014/jmb.2403.03039. Epub 2024 Apr 29.
The gastrointestinal (GI) tract of shrimp, which is comprised of the stomach, hepatopancreas, and intestine, houses microbial communities that play crucial roles in immune defense, nutrient absorption, and overall health. While the intestine's microbiome has been well-studied, there has been limited research investigating the stomach and hepatopancreas. The present study addresses this gap by profiling the bacterial community in these interconnected GI segments of Pacific whiteleg shrimp. To this end, shrimp samples were collected from a local aquaculture farm in South Korea, and 16S rRNA gene amplicon sequencing was performed. The results revealed significant variations in bacterial diversity and composition among GI segments. The stomach and hepatopancreas exhibited higher Proteobacteria abundance, while the intestine showed a more diverse microbiome, including Cyanobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Chloroflexi, and Verrucomicrobia. Genera such as , , , , and dominated the intestine, while , , and prevailed in the stomach and hepatopancreas. It is particularly notable that , which is associated with nitrate reduction and pollutant degradation, was prominent in the hepatopancreas. Overall, this study provides insights into the microbial ecology of the Pacific whiteleg shrimp's GI tract, thus enhancing our understanding of shrimp health with the aim of supporting sustainable aquaculture practices.
虾的胃肠道（GI）由胃、肝胰腺和肠组成，其中栖息着对免疫防御、营养吸收和整体健康至关重要的微生物群落。虽然肠道微生物组已经得到了充分研究，但对胃和肝胰腺的研究却很有限。本研究通过分析太平洋白对虾这些相互连接的 GI 段中的细菌群落来填补这一空白。为此，从韩国当地的一个水产养殖场收集了虾样本，并进行了 16S rRNA 基因扩增子测序。结果表明，GI 段之间的细菌多样性和组成存在显著差异。胃和肝胰腺表现出较高的变形菌丰度，而肠道则具有更丰富的微生物组，包括蓝细菌、放线菌、拟杆菌门、厚壁菌门、绿弯菌门和疣微菌门。属如、、、、和 主导着肠道，而 、 和 则在胃和肝胰腺中占优势。特别值得注意的是， ，它与硝酸盐还原和污染物降解有关，在肝胰腺中很突出。总的来说，本研究深入了解了太平洋白对虾 GI 道的微生物生态学，从而提高了我们对虾健康的理解，旨在支持可持续水产养殖实践。
