Bos G D, Goldberg V M, Gordon N H, Dollinger B M, Zika J M, Powell A E, Heiple K G
Clin Orthop Relat Res. 1985 Jul-Aug(197):245-54.
Fresh and frozen orthotopic iliac crest bone grafts in rats were studied histologically for determination of the long-term effects of histocompatibility matching and the freezing process on orthotopic bone graft incorporation. Grafts exchanged between groups of inbred rats, syngeneic or differing with respect to major or minor histocompatibility loci, were studied histologically at 20, 30, 40, 50, and 150 days after bone transplantation. A numerical histologic scoring system was developed and used by three observers for evaluation of coded hematoxylin and eosin sections. All frozen graft groups had the same fate regardless of histocompatibility relations between donors and recipients, and all grafts were inferior to fresh syngeneic grafts. Both fresh allograft groups received similar scores and initially at 20 and 30 days had scores similar to those of the fresh syngeneic groups. In the later intervals, however, the fresh allografts were inferior to the fresh syngeneic grafts and similar to the frozen groups. This is consistent with an older model describing two distinct phases of osteogenesis. In the long term, frozen syngeneic and fresh and frozen allografts across major and minor histocompatibility barriers were comparable, but all were significantly inferior to fresh syngeneic bone grafts.
对大鼠新鲜和冷冻的原位髂嵴骨移植进行组织学研究，以确定组织相容性匹配和冷冻过程对原位骨移植融合的长期影响。在骨移植后20、30、40、50和150天对近交系大鼠组之间交换的移植骨进行组织学研究，这些移植骨在主要或次要组织相容性位点上是同基因的或不同的。开发了一种数字组织学评分系统，并由三名观察者用于评估编码的苏木精和伊红切片。所有冷冻移植组的命运相同，与供体和受体之间的组织相容性关系无关，并且所有移植骨均不如新鲜的同基因移植骨。两个新鲜同种异体移植组的得分相似，最初在20天和30天时的得分与新鲜同基因组相似。然而，在后期，新鲜同种异体移植骨不如新鲜同基因移植骨，且与冷冻组相似。这与描述成骨两个不同阶段的一个较旧模型一致。从长期来看，跨越主要和次要组织相容性屏障的冷冻同基因移植骨以及新鲜和冷冻的同种异体移植骨具有可比性，但均明显不如新鲜同基因骨移植。
