Maeda A, Shino K, Horibe S, Matsumoto N, Nakamura N, Toritsuka Y
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
Clin Orthop Relat Res. 1997 Feb(335):298-309.
The differences in remodeling among frozen allografts, frozen autografts, and fresh autografts were investigated using a patellar tendon transplantation model in 2 different strains of rats. In this study, isografting (transplantation among the same strain inbred animals) was adopted as an autograft model. This method makes it possible to set up a frozen autograft model. Evaluation included mechanical properties determined during tensile failure tests, cross sectional area, histologic findings, and collagen fibril distribution. Recipient rats were euthanized at 4, 8, 12, and 24 weeks after transplantation (n = 5, in each group and in each period). The frozen allografts showed significantly less tensile strength than did the frozen autografts at 4 weeks, but this difference was transient and diminished by 8 weeks. The tensile strength of the fresh autografts was significantly greater than that of the frozen autografts at 8 weeks or the frozen allografts at 8 and 12 weeks. It was suggested that freeze thawing has adverse effects on the remodeling of fresh autografts in this extraarticular graft model. The cross sectional area for each type of graft at each posttransplantation period was about twice as large as that before transplantation. The collagen fibril profile of the frozen allografts was similar to that of the frozen autografts. However, the fresh autografts showed a greater number of large diameter fibrils and a lesser number of small diameter fibrils than did the other 2 frozen groups.
使用髌腱移植模型，在两种不同品系的大鼠中研究了冷冻同种异体移植物、冷冻自体移植物和新鲜自体移植物之间的重塑差异。在本研究中，采用同基因移植（同一品系近交动物之间的移植）作为自体移植模型。这种方法使得建立冷冻自体移植模型成为可能。评估包括拉伸破坏试验期间测定的力学性能、横截面积、组织学结果和胶原纤维分布。在移植后4周、8周、12周和24周对受体大鼠实施安乐死（每组和每个时间段n = 5）。在4周时，冷冻同种异体移植物的拉伸强度明显低于冷冻自体移植物，但这种差异是短暂的，到8周时减小。新鲜自体移植物在8周时的拉伸强度明显高于冷冻自体移植物，在8周和12周时高于冷冻同种异体移植物。提示在这种关节外移植模型中，冻融对新鲜自体移植物的重塑有不利影响。每个移植后时间段每种移植物的横截面积约为移植前的两倍。冷冻同种异体移植物的胶原纤维形态与冷冻自体移植物相似。然而，与其他两个冷冻组相比，新鲜自体移植物显示出更多大直径纤维和更少小直径纤维。
