Boz Valentina, Tesser Alessandra, Burlo Francesca, Donadel Nicola, Pastore Serena, Amaddeo Alessandro, Vittoria Francesca, Padovan Matteo, Di Rosa Marianna, Tommasini Alberto, Valencic Erica
Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy.
Department of Medical, Surgical and Health Sciences University of Trieste Trieste Italy.
Clin Transl Immunology. 2024 Jun 26;13(7):e1520. doi: 10.1002/cti2.1520. eCollection 2024.
Inflammatory markers such as erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are poorly informative about interferon (IFN)-related disorders. In these conditions, the measure of the interferon score (IS), obtained by measuring the expression of IFN-stimulated genes, has been proposed. Flow cytometry-based assays measuring sialic-acid-binding Ig-like lectin 1 (Siglec-1) expression could be a more practical tool for evaluating IFN-inflammation. The study compared Siglec-1 measures with IS and other inflammatory indexes. We compared Siglec-1 measures with IS and other inflammatory indexes in real-world paediatric rheumatology experience.
We recruited patients with immuno-rheumatological conditions, acute infectious illness and patients undergoing orthopaedic surgery as controls. Siglec-1 expression was measured in all samples, and IS, ESR and CRP were also recorded if available.
Overall, 98 subjects were enrolled in the study, with a total of 104 measures of Siglec-1. Compared with IS, Siglec-1 expression showed good accuracy (86.0%), specificity (72.7%) and sensitivity (85.7%). The measure of the percentage of Siglec-1-positive cells performed best at low levels of IFN-inflammation, while the measure of mean fluorescence intensity performed best at higher levels. studies on IFN-stimulated monocytes confirmed this behaviour. There was no link between Siglec-1 expression and either ESR or CRP, and positive Siglec-1 results were found even when ESR and CRP were normal. A high Siglec-1 expression was also recorded in subjects with acute infections.
Siglec-1 measurement by flow cytometry is an easy tool to detect IFN-related inflammation, even in subjects with normal results of common inflammation indexes.
红细胞沉降率（ESR）和C反应蛋白（CRP）等炎症标志物对于干扰素（IFN）相关疾病的诊断价值有限。在这些情况下，通过测量IFN刺激基因的表达来获得干扰素评分（IS）的方法被提出。基于流式细胞术检测唾液酸结合免疫球蛋白样凝集素1（Siglec-1）表达可能是评估IFN炎症的更实用工具。本研究比较了Siglec-1检测结果与IS及其他炎症指标。我们在实际的儿科风湿病诊疗中比较了Siglec-1检测结果与IS及其他炎症指标。
我们招募了免疫风湿性疾病患者、急性感染性疾病患者以及接受骨科手术的患者作为对照。检测了所有样本中Siglec-1的表达，如有可用数据，还记录了IS、ESR和CRP。
总体而言，98名受试者参与了本研究，共进行了104次Siglec-1检测。与IS相比，Siglec-1表达显示出良好的准确性（86.0%）、特异性（72.7%）和敏感性（85.7%）。Siglec-1阳性细胞百分比在低水平IFN炎症时表现最佳，而平均荧光强度在高水平时表现最佳。对IFN刺激的单核细胞的研究证实了这一现象。Siglec-1表达与ESR或CRP之间没有关联，即使ESR和CRP正常时也能检测到Siglec-1阳性结果。急性感染患者中也记录到高Siglec-1表达。
通过流式细胞术检测Siglec-1是一种简便的工具，即使在常见炎症指标结果正常的受试者中也能检测到IFN相关炎症。
