Crowley W R, Tessel R E, O'Donohue T L, Adler B A, Kalra S P
Endocrinology. 1985 Sep;117(3):1151-5. doi: 10.1210/endo-117-3-1151.
Recent pharmacological studies have suggested a role for neuropeptide Y (NPY) in control of LH secretion. The present study examined the effects of estradiol benzoate (EB) given alone or in combination with progesterone (P), on the concentrations of immunoreactive NPY in microdissected nuclei of the rat brain, in association with changes in LHRH concentrations and LH release. Forty-eight hours after the administration of EB to ovariectomized rats, serum LH was significantly reduced. Concentrations of NPY, measured by a sensitive and specific RIA, were also reduced in the median eminence, arcuate nucleus, and interstitial nucleus of the stria terminalis. As expected, administration of P to these EB-primed rats induced a sequential rise and fall of LHRH in the median eminence, followed by a marked LH surge in the afternoon. Interestingly, NPY concentrations in the median eminence also increased and then decreased after P, with a time course similar to that shown by LHRH. In the arcuate nucleus and interstitial nucleus of the stria terminalis, P treatment did not affect NPY concentrations further. Sequential EB plus P treatment significantly reduced NPY levels in the medial preoptic nucleus compared to oil vehicle controls. Levels of immunoreactive NPY in the ventromedial nucleus and periventricular nucleus were largely unaffected by ovarian hormone treatments. These results indicate that ovarian steroids, which modulate LH secretion, affect NPY concentrations in those specific areas of the brain which are known to be innervated by the LHRH neurons. These observations support the hypothesis that NPY may participate in the neural regulation of LHRH and LH secretion.
近期的药理学研究表明神经肽Y（NPY）在促黄体生成素（LH）分泌的调控中发挥作用。本研究检测了单独给予苯甲酸雌二醇（EB）或其与孕酮（P）联合使用时，对大鼠脑部分离核团中免疫反应性NPY浓度的影响，并同时观察促性腺激素释放激素（LHRH）浓度及LH释放的变化。给去卵巢大鼠注射EB 48小时后，血清LH显著降低。通过灵敏且特异的放射免疫分析（RIA）检测发现，正中隆起、弓状核及终纹间质核中的NPY浓度也降低。正如预期的那样，给这些经EB预处理的大鼠注射P后，正中隆起的LHRH先升高后降低，随后在下午出现明显的LH峰。有趣的是，正中隆起中的NPY浓度在注射P后也先升高后降低，其时间进程与LHRH相似。在弓状核和终纹间质核中，P处理未进一步影响NPY浓度。与油剂对照相比，序贯给予EB加P显著降低了视前内侧核中的NPY水平。腹内侧核和室旁核中的免疫反应性NPY水平在很大程度上不受卵巢激素处理的影响。这些结果表明，调节LH分泌的卵巢甾体激素会影响已知受LHRH神经元支配的脑特定区域中的NPY浓度。这些观察结果支持了NPY可能参与LHRH和LH分泌的神经调节这一假说。
