蛋白酶在大肠杆菌乳糖通透酶呼吸依赖性失活中的作用

Implication of proteases in the respiration dependent inactivation of the lactose permease of E. coli.

作者信息

Therisod H, Ghazi A, Shechter E

出版信息

FEBS Lett. 1985 Jul 22;187(1):38-42. doi: 10.1016/0014-5793(85)81209-6.

Abstract

The lactose permease of E. coli becomes irreversibly inactivated during lactose transport under conditions of high respiratory activity. This inactivation is characterized by a decrease in the steady state of lactose accumulation, a decrease in the influx rate of lactose, and a decrease in the transmembrane electrical potential. We report here that inhibitors of serine proteases (phenylmethylsulfonyl fluoride and N-alpha-P-tosyl-L-lysine chloromethyl ketone) prevent this inactivation, thus implicating proteases in this process.

摘要

在高呼吸活性条件下，大肠杆菌的乳糖通透酶在乳糖转运过程中会发生不可逆失活。这种失活的特征是乳糖积累的稳态下降、乳糖流入速率降低以及跨膜电势降低。我们在此报告，丝氨酸蛋白酶抑制剂（苯甲基磺酰氟和N-α-对甲苯磺酰-L-赖氨酸氯甲基酮）可防止这种失活，从而表明蛋白酶参与了这一过程。