Kisaoglu Aysegul, Kose Evren, Yilmaz Nesibe, Tanbek Kevser, Yildiz Azibe, Yilmaz Umit, Cirik Rumeyza Hilal, Ozbag Davut
Inonu University Faculty of Medicine, Department of Anatomy, Malatya, Türkiye.
Karabuk University Faculty of Medicine, Department of Anatomy, Karabuk, Türkiye.
Medeni Med J. 2024 Jun 28;39(2):101-108. doi: 10.4274/MMJ.galenos.2024.27243.
The aim of this study was to investigate the effect of various astaxanthin (ATX) doses on oxidative damage and autophagy in renal ischemia-reperfusion (I/R) injury-modeled rats.
The rats were divided into five groups: sham group (n=8), I/R (n=8), I/R + 5 mg/kg ATX (n=8), I/R + 10 mg/kg ATX (n=8), and I/R + 25 mg/kg ATX (n=8) groups. ATX was dissolved in 5 mg/kg, 10 mg/kg, and 25 mg/kg olive oil for 7 days and administered to the rats in the experimental group. Sham and I/R groups were also administered ATX solution (olive oil) via oral gavage for 7 days. Renal ischemia reperfusion was induced in all rats except the sham group after the last dose was administered on the 7 day. Reperfusion was conducted for 24 hours after 45 minutes of ischemia.
Blood samples were collected, and kidney tissue were incised for biochemical and histological analyses. Superoxide dismutase (SOD) and total antioxidant status (TAS) were significantly lower in the I/R group than in the sham group (p<0.05), whereas malondialdehyde (MDA) and total oxidant status (TOS) values were higher (p<0.05). It was determined that SOD and TAS increased and MDA and TOS decreased in the ATX-administration groups compared with the I/R group, independent of the dose (p<0.05). In the 25 mg/kg ATX + I/R group, Beclin-1 and LC3β immunoreactivities were significantly higher than those in the other groups (p<0.05). The lowest p62 immunoreactivity was observed in the 25 mg/kg ATX + I/R group.
ATX had a protective effect on kidney function and against oxidative damage. Furthermore, high-dose ATX administration protected kidney tissue via autophagy induction in this study.
本研究旨在探讨不同剂量虾青素（ATX）对肾缺血再灌注（I/R）损伤模型大鼠氧化损伤和自噬的影响。
将大鼠分为五组：假手术组（n = 8）、I/R组（n = 8）、I/R + 5 mg/kg ATX组（n = 8）、I/R + 10 mg/kg ATX组（n = 8）和I/R + 25 mg/kg ATX组（n = 8）。将ATX溶解于5 mg/kg、10 mg/kg和25 mg/kg的橄榄油中，连续7天给予实验组大鼠。假手术组和I/R组也通过灌胃给予ATX溶液（橄榄油）7天。在第7天最后一次给药后，除假手术组外，所有大鼠均诱导肾缺血再灌注。缺血45分钟后进行24小时再灌注。
采集血样，切开肾组织进行生化和组织学分析。I/R组超氧化物歧化酶（SOD）和总抗氧化状态（TAS）显著低于假手术组（p<0.05），而丙二醛（MDA）和总氧化状态（TOS）值较高（p<0.05）。与I/R组相比，ATX给药组的SOD和TAS升高，MDA和TOS降低，且与剂量无关（p<0.05）。在25 mg/kg ATX + I/R组中，Beclin-1和LC3β免疫反应性显著高于其他组（p<0.05）。在25 mg/kg ATX + I/R组中观察到最低的p62免疫反应性。
ATX对肾功能和氧化损伤具有保护作用。此外，在本研究中，高剂量ATX给药通过诱导自噬保护肾组织。
