Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" of Chieti, Italy.
Microbes Infect. 2024 Nov-Dec;26(8):105384. doi: 10.1016/j.micinf.2024.105384. Epub 2024 Jun 27.
Antimicrobial treatment of methicillin-resistant Staphylococcus pseudintermedius associated with canine wounds represents an important challenge. The aim of this study was to create a canine wound infection model, Lubbock Chronic Wound Biofilm (LCWB), with a focus on S. pseudintermedius, drawing inspiration from the established human model involving Staphylococcus aureus. Methicillin-resistant S. pseudintermedius 115 (MRSP) and Pseudomonas aeruginosa 700 strains, isolated from dog wounds, were used to set up the LCWB at 24, 48 and 72 h. The LCWBs were evaluated in terms of volume, weight, and microbial CFU/mg. The microbial spatial distribution in the LCWBs was assessed by SEM and CLSM imaging. The best incubation time for the LCWB production in terms of volume (3.38 cm ± 0.13), weight (0.86 gr ± 0.02) and CFU/mg (up to 7.05 × 10 CFU/mg ± 2.89 × 10) was 48 h. The SEM and CLSM images showed a major viable microbial colonization at 48 h with non-mixed bacteria with a prevalence of MRSP on the surface and P. aeruginosa 700 in the depth of the wound. The obtained findings demonstrate the capability of S. pseudintermedius to grow together P. aeruginosa in the LCWB model, representing the suitable model to reproduce the animal chronic wound in vitro.
耐甲氧西林金黄色葡萄球菌相关的犬创伤感染的抗菌治疗是一个重要的挑战。本研究旨在建立一个犬创伤感染模型，即拉伯克慢性创伤生物膜（LCWB），重点关注耐甲氧西林金黄色葡萄球菌（MRSP），灵感来自已建立的涉及金黄色葡萄球菌的人类模型。从犬创伤中分离出的耐甲氧西林金黄色葡萄球菌 115 （MRSP）和铜绿假单胞菌 700 株被用于在 24、48 和 72 小时建立 LCWB。通过体积、重量和微生物 CFU/mg 来评估 LCWB。通过 SEM 和 CLSM 成像评估 LCWB 中的微生物空间分布。从体积（3.38 cm ± 0.13）、重量（0.86 gr ± 0.02）和 CFU/mg（最高可达 7.05 × 10 CFU/mg ± 2.89 × 10）的角度来看，LCWB 生产的最佳孵育时间为 48 小时。SEM 和 CLSM 图像显示，在 48 小时时存在主要的存活微生物定植，表面以 MRSP 为主，深部为铜绿假单胞菌 700。研究结果表明，金黄色葡萄球菌能够与铜绿假单胞菌在 LCWB 模型中共同生长，这表明该模型适合在体外复制动物慢性创伤。
