Laboratório de Cocos Gram positivos, Instituto Biomédico, UFF, Brazil; Graduate Program in Veterinary Medicine, Fluminense Federal University, Brazil.
Laboratório de Cocos Gram positivos, Instituto Biomédico, UFF, Brazil; Graduate Program in Microbiology, Instituto de Microbiologia Paulo de Goes, UFRJ, Brazil.
J Infect Public Health. 2023 Dec;16 Suppl 1(Suppl 1):183-189. doi: 10.1016/j.jiph.2023.10.041. Epub 2023 Nov 3.
Dogs often carry methicillin-resistant Staphylococci asymptomatically. These bacteria are frequently linked to conditions such as canine pyoderma and otitis. Close interaction between dogs and humans can facilitate the exchange of resistant strains, particularly Methicillin-resistant Staphylococcus pseudintermedius (MRSP). This represents a public health issue, since these strains, in addition to occasionally causing infections in humans, can also serve as a source of resistance and virulence genes for strains of greater importance in human medicine, such as Staphylococcus aureus. Furthermore, MRSP strains are often multidrug resistant, which ends up compromising the treatment of infections. This study aimed to assess the potential transmission of Staphylococcus pseudintermedius among dogs and their owners. We examined a total of one hundred canine samples collected from cases of pyoderma and otitis to detect the presence of staphylococci. Simultaneously, we conducted evaluations on all dog owners. Staphylococci strains were identified using MALDI-TOF MS and PCR targeting the nuc gene. Methicillin resistance screening was also performed by detecting the mecA gene using PCR. Among the sampled dogs, 64 carried S. pseudintermedius. Nine were identified as MRSP. In six instances, dogs and their owners exhibited S. pseudintermedius. These samples underwent genome sequencing and were screened for antimicrobial resistance genes, SCCmec typing, MLST characterization, and Single Nucleotide Polymorphisms (SNP) analyses. The results of the phylogenetic analysis revealed that in three cases, dogs and owners had closely related isolates, suggesting interspecies transmission. Two of these cases involved MRSP and one MSSP. Moreover, in the two MRSP cases, the same SCCmec type (type V) was detected. Additionally, the sequence type was consistent across all three cases involving dogs and owners (MSSP ST2277, MRSP ST2282, and ST2286). These findings strongly indicate a transmission event. Since Staphylococcus pseudintermedius is primarily isolated from canine samples, it is plausible that dogs may have acted as a potential source. In the remaining three cases, despite identifying the same species in both samples, they had notable phylogenetic differences.
狗常无症状携带耐甲氧西林葡萄球菌。这些细菌常与犬脓皮病和耳炎等疾病相关。狗和人类的密切接触可促进耐药株的交换，尤其是耐甲氧西林中间葡萄球菌（MRSP）。这是一个公共卫生问题，因为这些菌株除了偶尔会导致人类感染外，还可以作为人类医学中更重要的菌株（如金黄色葡萄球菌）的耐药性和毒力基因的来源。此外，MRSP 菌株通常为多药耐药株，这最终会影响感染的治疗。本研究旨在评估中间葡萄球菌在犬及其主人之间的潜在传播。我们共检查了 100 份来自脓皮病和耳炎病例的犬样本，以检测葡萄球菌的存在。同时，我们对所有犬主人进行了评估。使用 MALDI-TOF MS 和针对 nuc 基因的 PCR 鉴定葡萄球菌株。通过检测 mecA 基因进行耐甲氧西林筛选。在所采样的犬中，64 只携带中间葡萄球菌。其中 9 株被鉴定为 MRSP。在 6 例中，犬及其主人表现出中间葡萄球菌。这些样本进行了基因组测序，并对其进行了抗生素耐药基因、SCCmec 分型、MLST 特征和单核苷酸多态性（SNP）分析的筛选。系统发育分析的结果表明，在 3 例中，犬和主人的分离株具有密切相关的亲缘关系，提示存在种间传播。其中两例涉及 MRSP，一例 MSSP。此外，在两例 MRSP 病例中，检测到相同的 SCCmec 型（V 型）。此外，所有涉及犬和主人的三个病例的序列型均一致（MSSP ST2277、MRSP ST2282 和 ST2286）。这些发现强烈表明存在传播事件。由于中间葡萄球菌主要从犬样本中分离，因此犬可能是潜在的来源。在其余 3 例中，尽管在两个样本中都鉴定出相同的物种，但它们具有显著的系统发育差异。
