Shimokawa H, Tomoike H, Nabeyama S, Yamamoto H, Nakamura M
J Am Coll Cardiol. 1985 Aug;6(2):321-7. doi: 10.1016/s0735-1097(85)80167-4.
The role of prostanoids in a swine model of coronary artery spasm was examined. Eighteen miniature pigs underwent endothelial denudation of the left coronary artery (left circumflex branch in 14 pigs and left anterior descending branch in 4 pigs) followed by high cholesterol feeding. Three months after the denudation, when coronary artery spasm was repeatedly provoked along the denuded portion of the coronary artery by histamine, the vasoconstrictive effect of thromboxane A2 and the preventive effects of indomethacin and prostacyclin against histamine-induced coronary artery spasm were examined. Intracoronary administration of thiothromboxane A2, 200 micrograms, a stable thromboxane A2 analog, failed to provoke coronary artery spasm (seven of seven cases) but nonselectively constricted the coronary artery by 33%. Intravenous administration of indomethacin, 2 mg/kg, or continuous intravenous infusion of prostacyclin, 50 ng/kg per min, failed to prevent histamine-induced coronary artery spasm (four of four and eight of eight cases, respectively), yet the spasm was all but prevented by intravenous pretreatment with diphenhydramine at a dose of 1 mg/kg. Thus, in this swine model, prostanoids may not play a primary role in the occurrence of coronary artery spasm.
研究了类前列腺素在猪冠状动脉痉挛模型中的作用。18只小型猪接受了左冠状动脉内皮剥脱术（14只猪为左旋支，4只猪为左前降支），随后给予高胆固醇饮食。内皮剥脱术后3个月，当通过组胺反复刺激冠状动脉剥脱部位诱发冠状动脉痉挛时，检测了血栓素A2的血管收缩作用以及吲哚美辛和前列环素对组胺诱导的冠状动脉痉挛的预防作用。冠状动脉内注射200微克硫代血栓素A2（一种稳定的血栓素A2类似物）未能诱发冠状动脉痉挛（7例均未出现），但非选择性地使冠状动脉收缩了33%。静脉注射2毫克/千克吲哚美辛或持续静脉输注50纳克/千克每分钟前列环素均未能预防组胺诱导的冠状动脉痉挛（分别为4例中的4例和8例中的8例），然而，静脉注射1毫克/千克苯海拉明预处理几乎可以预防痉挛。因此，在这个猪模型中，类前列腺素可能在冠状动脉痉挛的发生中不发挥主要作用。
