Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University.
Agricultural Technology and Innovation Research Institute, Kindai University.
J Nutr Sci Vitaminol (Tokyo). 2024;70(3):262-272. doi: 10.3177/jnsv.70.262.
Osteoporosis is characterized by bone loss and deterioration in bone microstructure, leading to bone fragility. It is strongly correlated with menopause in women. Previously, we reported that diets supplemented with a kudzu (Pueraria lobata) vine extract suppressed bone resorption in ovariectomized (OVX) mice, a postmenopausal model. The main isoflavone in kudzu is puerarin (daidzein-8-C-glycoside). Puerarin (daidzein-8-C-glycoside), which is main isoflavone of kudzu, probably contributes to the beneficial effect. However, the underlying mechanism is unclear. Therefore, the nutrikinetics of puerarin and the comparison with the suppressive effects of kudzu isoflavones on osteoclast differentiation was examined in this study. We demonstrated that orally administered puerarin was absorbed from the gut and entered the circulation in an intact form. In addition, puerarin accumulated in RAW264.7 pre-osteoclast cells in a time-dependent manner. Tartrate-resistant acid phosphatase activity was decreased by puerarin treatment in a concentration-dependent manner in RAW264.7 cells stimulated with the receptor activator of nuclear factor kappa-B ligand. Ovariectomy-induced elevated bone resorption was suppressed, and the fragile bone strength was improved by puerarin ingestion in the diet. These findings suggested that orally administered puerarin was localized in bone tissue and suppressed bone resorption and osteoclastogenesis in ovariectomized mice.
骨质疏松症的特征是骨量流失和骨微观结构恶化，导致骨骼脆弱。它与女性绝经密切相关。之前，我们报道了补充葛藤（Pueraria lobata）藤提取物的饮食可以抑制去卵巢（OVX）小鼠（绝经后模型）的骨吸收。葛藤的主要异黄酮是葛根素（daidzein-8-C-糖苷）。葛根素（daidzein-8-C-糖苷），作为葛藤的主要异黄酮，可能对有益作用有贡献。然而，其潜在机制尚不清楚。因此，本研究检查了葛根素的营养动力学及其与葛藤异黄酮对破骨细胞分化的抑制作用的比较。我们证明了口服葛根素可以从肠道吸收并以完整形式进入循环。此外，葛根素在 RAW264.7 前破骨细胞中以时间依赖性方式积累。葛根素处理以浓度依赖性方式降低了 RAW264.7 细胞中核因子 kappa-B 受体激活剂配体刺激的抗酒石酸酸性磷酸酶活性。葛根素摄入饮食可抑制去卵巢引起的骨吸收增加，并改善脆弱的骨强度。这些发现表明，口服葛根素定位于骨组织，并抑制去卵巢小鼠的骨吸收和破骨细胞生成。
