Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, 3327-204 Naka-machi, Nara 631-8505, Japan.
J Agric Food Chem. 2011 Dec 28;59(24):13230-7. doi: 10.1021/jf2031617. Epub 2011 Nov 18.
Bone-loss-improving action of kudzu vine ethanol extracts (PVEE) was clarified. PVEE was composed roughly of 80% fiber, 10% puerarin, 3.6% daidzin, 2.5% 6″-O-malonyldaidzin, and the other minor isoflavones. Ten-week-old ovariectomized (OVX) mice were fed diets containing PVEE (20 mg/kg body weight/day) for 8 weeks. The bone resorption markers (urinary deoxypyridinoline and tartrate-resistant acid phosphatase activity) was elevated in OVX mice and was significantly decreased in OVX mice that consumed PVEE for 8 weeks. Consistent with the decrease in the markers, the number of matured osteoclasts in the distal femur was diminished in OVX mice fed PVEE diets. PVEE diets also suppressed the decrease in femoral bone mineral density (BMD) by OVX. PVEE showed the affinity for estrogen receptor α and β nearly 1/10000 weaker than 17β-estradiol. No hypertrophy in the uterus by the PVEE diet was observed. These results suggest that PVEE could be a promising resource for a functional food that improves osteoporosis.
阐明了野葛乙醇提取物（PVEE）的改善骨质流失作用。PVEE 大致由 80%纤维、10%葛根素、3.6%大豆苷、2.5%6″-O-乙酰基黄豆苷元和其他少量异黄酮组成。10 周龄去卵巢（OVX）小鼠喂食含 PVEE（20 mg/kg 体重/天）的饮食 8 周。骨吸收标志物（尿脱氧吡啶啉和耐酒石酸酸性磷酸酶活性）在 OVX 小鼠中升高，而在喂食 8 周 PVEE 的 OVX 小鼠中则显著降低。与标志物的减少一致，在喂食 PVEE 饮食的 OVX 小鼠的远端股骨中，成熟破骨细胞的数量减少。PVEE 饮食还抑制了 OVX 引起的股骨骨密度（BMD）下降。PVEE 对雌激素受体 α 和 β 的亲和力比 17β-雌二醇弱约 1/10000。未观察到 PVEE 饮食引起子宫肥大。这些结果表明，PVEE 可能是一种有前途的功能性食品资源，可以改善骨质疏松症。
