Chouhan Devendra Kumar, Patel Sandeep, Thami Tarkik, Mishra Narayan Prasad, Nahar Uma, Dhillon Mandeep Singh
Department of Orthopedic Surgery, Post Graduate Institute of Medical Education & Research (PGIMER), Gate No. 15, Chandigarh, 160012 India.
Department of Orthopedic Surgery, All India Institute of Medical Sciences (AIIMS), Jodhpur, 342001 India.
Indian J Orthop. 2024 Apr 19;58(7):887-893. doi: 10.1007/s43465-024-01145-z. eCollection 2024 Jul.
The evaluation of anti-apoptotic and chondroprotective properties of a single injection of PRP using immunohistochemistry (IHC).
This was a placebo-controlled blinded experimental study. Ten healthy Dunkin Hartley guinea pigs were selected. One knee of each animal was injected with a single injection of PRP (Group A); the contralateral knee acted as a control and was injected with a single injection of normal saline (Group B). These groups were further divided into A3 and B3 based on the timeline of animal sacrifice (3 months) and A6 and B6 (6 months). The formalin-preserved articular cartilage blocks were subjected to IHC to stain Aggrecan, Caspase-3, and Collagen-2.
The mean IHC score was significantly low for Caspase-3 (-0.029) in intervention group (A3) in comparison to placebo control group (B3) pointing towards decreased apoptosis. The mean IHC values were significantly higher for Collagen II (-0.011) for intervention group (A6) in contrast to control group (B6); values were also significantly low for Caspase-3 (-0.029) in A6 as compared to B6. The mean Caspase-3 values were significantly higher in A6 as compared to A3 (-0.029).
The impact of a solitary injection of PRP on upregulation of anabolic pathways inside cartilage is relatively slower as compared to its effect on downregulation of apoptotic pathways. Even a single PRP injection holds the potential to change cartilage microenvironment, but the effects are not long lasting.
使用免疫组织化学（IHC）评估单次注射富血小板血浆（PRP）的抗凋亡和软骨保护特性。
这是一项安慰剂对照的双盲实验研究。选择了10只健康的邓金·哈特利豚鼠。每只动物的一个膝关节单次注射PRP（A组）；对侧膝关节作为对照，单次注射生理盐水（B组）。根据动物处死时间（3个月），这些组进一步分为A3和B3组，以及A6和B6组（6个月）。对用福尔马林保存的关节软骨块进行免疫组织化学染色，以检测聚集蛋白聚糖、半胱天冬酶-3和胶原蛋白-2。
与安慰剂对照组（B3）相比，干预组（A3）中半胱天冬酶-3的平均免疫组织化学评分显著较低（-0.029），表明细胞凋亡减少。与对照组（B6）相比，干预组（A6）中胶原蛋白II的平均免疫组织化学值显著较高（-0.011）；与B6相比，A6中半胱天冬酶-3的值也显著较低（-0.029）。与A3（-0.029）相比，A6中的半胱天冬酶-3平均值得显著更高。
与对凋亡途径下调的作用相比，单次注射PRP对软骨内合成代谢途径上调的影响相对较慢。即使单次注射PRP也有可能改变软骨微环境，但其效果并不持久。
