Asjid Rafia, Faisal Tayyaba, Qamar Khadija, Malik Sana, Umbreen Faiza, Fatima Mahjabeen
Department of Anatomy, Army Medical College, National University of Medical Sciences, Rawalpindi, Pakistan.
Department of Anatomy, Federal Medical and Dental College, Islamabad.
J Coll Physicians Surg Pak. 2019 Nov;29(11):1067-1071. doi: 10.29271/jcpsp.2019.11.1067.
To evaluate the effects of platelet-rich plasma injection on histological changes of osteoarthritis using the modified Mankin score in monoiodoacetate-induced rat knee model.
Laboratory-based experimental study.
Department of Anatomy, Army Medical College, Rawalpindi and National Institute of Health (NIH), Islamabad from March to May 2018.
Thirty-two pathogen-free male rats aged 3-4 months were selected and divided into two groups with 8 and 24 animals, respectively. Groups A was used as control. Experimental group B was further subgrouped as B1, B2 and B3 with 8 animals, in each subgroup. Monoiodoacetate solution was injected into the articular cavity of the right knee joint of all the animals in group B. Both the control (A) and experimental group B1 were sacrificed after 2 weeks of MIA injection, for observing the histological changes in the articular cartilage. Group B2 received a single injection of platelet rich plasma (PRP) while group B3 was reared as such. Both groups were sacrificed after 4 weeks of intra-articular administration of PRP. Articular cartilage was collected, processed and stained with hematoxylin and eosin (H and E) and toludine blue (T.B). Routine histological study was done and Mankin score was calculated to determine the grade of arthritis. SPSS Version 21 was used for the statistical analysis and p-value was considered significant at the cut-off value of 0.05.
The mean Mankin score of group B3 (9.37) was significantly higher than that of group B2 (7.37, p=0.003).
Platelet-rich plasma treatment controls the histological changes of chemically-induced osteoarthritis in rat knee.
在单碘乙酸诱导的大鼠膝关节模型中，使用改良的曼金评分来评估富血小板血浆注射对骨关节炎组织学变化的影响。
基于实验室的实验研究。
2018年3月至5月，位于拉瓦尔品第的陆军医学院解剖学系和伊斯兰堡的国家卫生研究所（NIH）。
选取32只3 - 4个月龄的无特定病原体雄性大鼠，分为两组，分别为8只和24只。A组作为对照组。实验组B进一步分为B1、B2和B3三个亚组，每组8只动物。向B组所有动物的右膝关节腔注射单碘乙酸溶液。在注射单碘乙酸2周后，处死对照组（A）和实验组B1，以观察关节软骨的组织学变化。B2组接受单次富血小板血浆（PRP）注射，而B3组则按原样饲养。在关节腔内注射PRP 4周后，处死两组动物。收集关节软骨，进行处理，并用苏木精和伊红（H&E）以及甲苯胺蓝（T.B）染色。进行常规组织学研究并计算曼金评分以确定关节炎的等级。使用SPSS 21版进行统计分析，p值在0.05的临界值时被认为具有显著性。
B3组的平均曼金评分（9.37）显著高于B2组（7.37，p = 0.003）。
富血小板血浆治疗可控制大鼠膝关节化学诱导性骨关节炎的组织学变化。
