Ntampakis Georgios, Pramateftakis Manousos-Georgios, Anestiadou Elissavet, Bitsianis Stefanos, Ioannidis Orestis, Bekiari Chryssa, Koliakos George, Karakota Maria, Tsakona Anastasia, Cheva Angeliki, Angelopoulos Stamatios
Fourth Department of Surgery, Medical School, Aristotle University of Thessaloniki, Thessaloniki 57010, Greece.
Laboratory of Anatomy and Histology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
World J Exp Med. 2024 Jun 20;14(2):94135. doi: 10.5493/wjem.v14.i2.94135.
Anastomotic leaks remain one of the most dreaded complications in gastrointestinal surgery causing significant morbidity, that negatively affect the patients' quality of life. Experimental studies play an important role in understanding the pathophysiological background of anastomotic healing and there are still many fields that require further investigation. Knowledge drawn from these studies can lead to interventions or techniques that can reduce the risk of anastomotic leak in patients with high-risk features. Despite the advances in experimental protocols and techniques, designing a high-quality study is still challenging for the investigators as there is a plethora of different models used.
To review current state of the art for experimental protocols in high-risk anastomosis in rats.
This systematic review was performed according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. To identify eligible studies, a comprehensive literature search was performed in the electronic databases PubMed (MEDLINE) and Scopus, covering the period from conception until 18 October 2023.
From our search strategy 102 studies were included and were categorized based on the mechanism used to create a high-risk anastomosis. Methods of assessing anastomotic healing were extracted and were individually appraised.
Anastomotic healing studies have evolved over the last decades, but the findings are yet to be translated into human studies. There is a need for high-quality, well-designed studies that will help to the better understanding of the pathophysiology of anastomotic healing and the effects of various interventions.
吻合口漏仍然是胃肠外科手术中最可怕的并发症之一，会导致严重的发病率，对患者的生活质量产生负面影响。实验研究在理解吻合口愈合的病理生理背景方面发挥着重要作用，仍有许多领域需要进一步研究。从这些研究中获得的知识可以带来干预措施或技术，降低具有高危特征患者吻合口漏的风险。尽管实验方案和技术取得了进展，但由于使用了大量不同的模型，设计高质量的研究对研究人员来说仍然具有挑战性。
综述大鼠高危吻合口实验方案的当前技术水平。
本系统评价按照系统评价和Meta分析的首选报告项目指南进行。为了识别 eligible 研究，在电子数据库PubMed（MEDLINE）和Scopus中进行了全面的文献检索，涵盖从 conception 到2023年10月18日的时间段。
根据我们的检索策略，纳入了102项研究，并根据用于创建高危吻合口的机制进行了分类。提取了评估吻合口愈合的方法并进行了单独评估。
在过去几十年中，吻合口愈合研究不断发展，但研究结果尚未转化为人体研究。需要高质量、精心设计的研究，以帮助更好地理解吻合口愈合的病理生理学以及各种干预措施的效果。
